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Merck
CN

03-0199-00

SMC® Human NPTX2 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human NPTX2 in human serum, plasma and cerebrospinal fluid (CSF)

Synonym(s):

NPTX2 high sensitive immunoassay, NPTX2 single molecule counting kit, Neuronal pentraxin 2 immuno assay kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
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product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

manufacturer/tradename

SMC®

assay range

sensitivity: 0.58 pg/mL
(Lower Limit of Quantification)

inter-assay cv: 5%
intra-assay cv: 6%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days)

technique(s)

single molecule counting: suitable

input

cerebrospinal fluid (CSF)
serum
plasma (K2 EDTA)

UniProt accession no.

compatibility

for use with FemtoQuest and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

Neuronal Pentraxin 2 (NPTX2) actively contributes to synaptic function and plasticity in the brain. As a member of the pentraxin family, NPTX2 regulates synaptic transmission and clears excess neurotransmitters. Predominantly expressed in neurons, it facilitates the formation and maintenance of synapses, playing a crucial role in learning and memory. NPTX2 has been linked to conditions such as Alzheimer′s disease and schizophrenia. Altered levels of NPTX2 correlate with synaptic dysfunction and cognitive decline, suggesting its potential as a biomarker for these diseases. Additionally, NPTX2 influences neuroinflammatory processes by affecting interactions between neurons and glial cells. Understanding NPTX2′s function and its impact on synaptic integrity and neuroinflammation is essential for developing therapeutic strategies that aim to preserve cognitive function and mitigate the effects of neurodegeneration in various neurological disorders.

Application

NeuroscienceNeurological DisordersNeuronal & Glial Markers

Biochem/physiol Actions

Although NPTX2 was detectable in non-human primate (rhesus macaque and cynomolgus monkey), feline, rat, mouse, and canine samples, the assay has not been verified for these species.

Features and Benefits

  • Ultrasensitive Detection: Detects NPTX2 at femtogram per milliliter levels in serum, plasma and CSF.
  • Improved Signal Fidelity: Expanded dynamic range and higher signal-to-noise deliver consistent, accurate results.
  • Familiar Sandwich Format: Uses a standard sandwich immunoassay that ELISA-trained labs can adopt easily while maintaining high sensitivity for Aβ1-42.
  • Automation-compatible Design: Engineered for seamless integration with automated liquid-handling platforms to streamline workflows and reduce manual steps. Dedicated Technical Support: Backed by experts experienced in SMC® assay technology.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 580 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 290-1,000,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 93% Plasma: 104% CSF: 97%
  • Recovery: Serum: 99% Plasma: 104% CSF: 99%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.
NeuroscienceNeurological DisordersNeuronal & Glial Markers

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Regulatory Information

新产品
This item has

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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