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03-0208-00

SMC® Human PD-L1 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human PD-L1 in human serum and plasma

Synonym(s):

PD-L1 ultra-sensitive immunoassay, PD-L1 immunoassay kit, Programmed cell death protein ligand 1 kit, PD-L1 kit, Single Molecule Counting, SMC, SMC®, PD-L1, Human PD-L1, Hu PD-L1, checkpoint protein PD-L1, Immuno-oncology PD-L1, PD-L1 Single Molecule Counting kit

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About This Item

NACRES:
NA.51
UNSPSC Code:
12161503
Species reactivity:
human
Technique(s):
single molecule counting: suitable
Usage:
 kit sufficient for 96 wells
Compatibility:
for use with FemtoQuest™ and SMCxPRO® instruments
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Product Name

SMC® Human PD-L1 High Sensitivity Immunoassay Kit,

Quality Segment

200

description

Used for ultrasensitive quantification of Human PD-L1 in human serum and plasma

product line

SMC®

usage

 kit sufficient for 96 wells

species reactivity

human

technique(s)

single molecule counting: suitable

input

plasma (K2 EDTA)
serum

UniProt accession no.

Q9NZQ7

compatibility

for use with FemtoQuest™ and SMCxPRO® instruments

shipped in

wet ice

storage temp.

2-8°C

General description

Programmed cell death protein ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) and B7-H1, is a type 1 membrane protein of 290 amino acids. It is found in a variety of cell types and binds programmed cell death protein (PD-1). It has been indicated as playing a role in autoimmune disease, allograft responses, pregnancy, and other disease states. When PD-L1 binds to PD-1, an inhibitory signal is transmitted that ultimately reduces the proliferation of antigen-specific T cells and reduces the rate of apoptosis in regulatory T-cells. By doing so, PD-L1 contributes to the immune evasion of cancer tissues by suppressing the adaptive immune system. Blocking this activity is thought to restore anti-tumor activity. High PD-L1 expression is correlated with poor prognosis with some cancer types. High levels of the soluble form are also associated with poor prognosis and advanced tumor progression. Multiple PD-L1 inhibiting monoclonal antibodies have been approved as cancer therapeutics.

Application

Immuno-Oncology

Features and Benefits

  • Unlock Hidden Insights in Cancer Immunology: Detect soluble PD-L1 levels associated with tumor progression and poor prognosis, even at sub-picogram concentrations.
  • Accelerate Biomarker Discovery: Ideal for studying immune checkpoint dynamics in cancer, autoimmune disease, and transplant biology.
  • Confidence in Every Data Point: High recovery rates and linearity ensure data integrity across sample types. SMC® T
  • Technology: Digitally detects PD-L1 at ultrasensitive levels—down to 780 fg/mL, ideal for low-expression biomarker studies.

Packaging

96 well assay

Analysis Note

  • Sensitivity: LLOQ 780 fg/mL* (SMCxPRO® Instrument)
  • Standard Curve Range: 390-400,000 fg/mL
  • Intra-assay CV: <15%
  • Inter-assay CV: <20%
  • Linearity: Serum: 120% Plasma: 110%
  • Recovery: Serum: 117% Plasma: 109%
*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although PD-L1 was detectable in rat, mouse, monkey, and canine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany
SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

target_organs

Respiratory Tract

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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