Skip to Content
Merck
CN

04-035

Anti-PTEN Antibody, clone 6H2.1

clone 6H2.1, Upstate®, from mouse

Synonym(s):

Anti-PTEN antibody

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Anti-PTEN Antibody, clone 6H2.1, clone 6H2.1, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6H2.1, monoclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_000305.3

UniProt accession no.

O08586
P60484

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PTEN(5728), TEP1(7011)

Analysis Note

routinely evaluated on HeLa cell lysate

Application

Anti-PTEN Antibody, clone 6H2.1 detects level of PTEN & has been published & validated for use in WB, IP, IH(P).
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Biochem/physiol Actions

recognizes PTEN

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

55 kDa
The protein and lipid phosphatase PTEN (phosphatase and tensin homolog deleted on chromosome ten) has been suggested to play a role in tumorgenesis. PTEN is a tumor suppressor whose loss permits constitutive signaling through the PI3K pathway and is sufficient to cause cancer. In normal cells, in conjunction with FOXO1A and PTEN function together to restrain cell growth. PTEN metabolizes the product of PI3K phosphorylation of PI(4,5)P2 following receptor activation, creating PI(3,4,5)P3. In cells lacking PTEN, there are elevated levels of PI(3,4,5)P3, which acts as a potent second messenger promoting oncogenesis. Restoration of PTEN function is considered a novel approach to cancer therapy. Overexpression of PTEN inhibits cell migration, and expression of antisense PTEN mRNA enhances migration.

Immunogen

C-terminal 100 amino acids of PTEN
Epitope: C-terminal region

Other Notes

Replaces: 07-016

Physical form

Format: Purified
Protein G Purified
Purified mouse IgG in storage buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, with 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Whole chromosome instability caused by Bub1 insufficiency drives tumorigenesis through tumor suppressor gene loss of heterozygosity.
Baker, DJ; Jin, F; Jeganathan, KB; van Deursen, JM
Cancer Cell null
Role of DNA methylation and epigenetic silencing of HAND2 in endometrial cancer development.
Jones, A; Teschendorff, AE; Li, Q; Hayward, JD; Kannan, A; Mould, T; West, J; Zikan et al.
PLoS Medicine null
Peptide inhibitors disrupt the serotonin 5-HT2C receptor interaction with phosphatase and tensin homolog to allosterically modulate cellular signaling and behavior.
Anastasio, NC; Gilbertson, SR; Bubar, MJ; Agarkov, A; Stutz, SJ; Jeng, Y; Bremer et al.
The Journal of Neuroscience null
Yuh-Shyan Tsai et al.
Cancer science, 110(4), 1208-1219 (2019-02-06)
Prothymosin-α (PTMA) is a small, acidic protein that is usually transported into the nucleus and involves many cellular and immunological functions. Previous studies demonstrated that aberrant location of PTMA expression exists in human bladder cancer, but the role of PTMA
Dimitri G Trembath et al.
Melanoma research, 31(3), 264-267 (2021-04-20)
PTEN and p16 frequently undergo (epi)genetic aberrations in melanoma resulting in decreased, or absent, protein levels. We investigated the prognostic significance of these tumor suppressor genes in melanoma brain metastases (MBMs). Immunohistochemical analysis was performed on archived tissue sections from
View All Related Papers

Related Content

Western Blotting Tools

Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service