04-037
Anti-RalB Antibody, clone 25
clone 25, Upstate®, from mouse
Synonym(s):
RAS-like protein B, v-ral simian leukemia viral oncogene homolog B, GTP binding protein, v-ral simian leukemia viral oncogene homolog B
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
25, monoclonal
Application:
WB
Citations:
17
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
25, monoclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... RALB(5899)
General description
24 kDa
RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarizatied exocystossis in epithelaial cells (Cascone, 2008). RalB is required for metastatic formation and cell survival by driving exocyst assembly during polarized cell migration (Cascone, 2008). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes
Immunogen
Epitope: C-terminus
Synthetic peptide of the C-terminus of RalB
Application
Detect RalB using this Anti-RalB Antibody, clone 25 validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
G-proteins
Cytoskeletal Signaling
G-proteins
Biochem/physiol Actions
Other species have not been tested.
This antibody is specific for RalB and does not detect RalA.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Analysis Note
Evaluated by western blot on HEK293 or NIH/3T3 cell lysates.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Sarah R Pollock et al.
PloS one, 14(4), e0214764-e0214764 (2019-04-18)
Healthy mitochondria use an electrochemical gradient across the inner mitochondrial membrane (IMM) to generate energy in the form of ATP. A variety of endogenous and exogenous factors can lead to transient or sustained depolarization of the IMM, including mitochondrial fission
RalA but not RalB enhances polarized delivery of membrane proteins to the basolateral surface of epithelial cells.
Shipitsin, Michail and Feig, Larry A
Molecular and cellular biology, 24, 5746-5756 (2004)
Yuchen Chien et al.
EMBO reports, 4(8), 800-806 (2003-07-12)
The monomeric RAL (RAS-like) GTPases have been indirectly implicated in mitogenic regulation and cell transformation. Here, we show that RALA and RALB collaborate to maintain tumorigenicity through regulation of both proliferation and survival. Remarkably, this task is divided between these
Hua Yang et al.
The Journal of biological chemistry, 295(10), 3055-3063 (2020-02-01)
In human cancer cells that harbor mutant KRAS and WT p53 (p53), KRAS contributes to the maintenance of low p53 levels. Moreover, KRAS depletion stabilizes and reactivates p53 and thereby inhibits malignant transformation. However, the mechanism by which KRAS regulates
Richard R Clough et al.
The Journal of biological chemistry, 277(32), 28972-28980 (2002-05-30)
Ral GTPases may be involved in calcium/calmodulin-mediated intracellular signaling pathways. RalA and RalB are activated by calcium, and RalA binds calmodulin in vitro. It was examined whether RalA can bind calmodulin in vivo, whether RalB can bind calmodulin, and whether
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