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04-037

Sigma-Aldrich

Anti-RalB Antibody, clone 25

clone 25, Upstate®, from mouse

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Synonym(s):
RAS-like protein B, v-ral simian leukemia viral oncogene homolog B, GTP binding protein, v-ral simian leukemia viral oncogene homolog B
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

25, monoclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_002881.2

UniProt accession no.

P11234

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RALB(5899)

General description

RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarizatied exocystossis in epithelaial cells (Cascone, 2008). RalB is required for metastatic formation and cell survival by driving exocyst assembly during polarized cell migration (Cascone, 2008). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes

Specificity

Other species have not been tested.
This antibody is specific for RalB and does not detect RalA.

Immunogen

Epitope: C-terminus
Synthetic peptide of the C-terminus of RalB

Application

Detect RalB using this Anti-RalB Antibody, clone 25 validated for use in WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

G-proteins

Quality

Evaluated by western blot on HEK293 or NIH/3T3 cell lysates.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect RalB in HEK293, NIH/3T3, and rat liver cell lysate.

Target description

24 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Sarah R Pollock et al.
PloS one, 14(4), e0214764-e0214764 (2019-04-18)
Healthy mitochondria use an electrochemical gradient across the inner mitochondrial membrane (IMM) to generate energy in the form of ATP. A variety of endogenous and exogenous factors can lead to transient or sustained depolarization of the IMM, including mitochondrial fission
Yuchen Chien et al.
EMBO reports, 4(8), 800-806 (2003-07-12)
The monomeric RAL (RAS-like) GTPases have been indirectly implicated in mitogenic regulation and cell transformation. Here, we show that RALA and RALB collaborate to maintain tumorigenicity through regulation of both proliferation and survival. Remarkably, this task is divided between these
Hua Yang et al.
The Journal of biological chemistry, 295(10), 3055-3063 (2020-02-01)
In human cancer cells that harbor mutant KRAS and WT p53 (p53), KRAS contributes to the maintenance of low p53 levels. Moreover, KRAS depletion stabilizes and reactivates p53 and thereby inhibits malignant transformation. However, the mechanism by which KRAS regulates
RalA but not RalB enhances polarized delivery of membrane proteins to the basolateral surface of epithelial cells.
Shipitsin, Michail and Feig, Larry A
Molecular and cellular biology, 24, 5746-5756 (2004)
Carine Rossé et al.
Molecular and cellular biology, 26(2), 727-734 (2005-12-31)
The Ras family GTPases RalA and RalB have been defined as central components of the regulatory machinery supporting tumor initiation and progression. Although it is known that Ral proteins mediate oncogenic Ras signaling and physically and functionally interact with vesicle
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