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Merck
CN

04-135

Anti-Brachyury Antibody, clone 3E4.2

clone 3E4.2, Upstate®, from mouse

Synonym(s):

T brachyury (mouse) homolog, T brachyury-like, T protein, T, brachyury homolog (mouse), transcription factor T

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Brachyury Antibody, clone 3E4.2, clone 3E4.2, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3E4.2, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

input

sample type mesenchymal stem cell(s)

isotype

IgG1κ

NCBI accession no.

NP_003172

UniProt accession no.

O15178

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TBXT(6862)

Analysis Note

Routinely evaluated by Western blot on NT2 cell lysates.

Western Blotting:
Recommended working dilutions are 1:1000.

Application

Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
This Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.

Biochem/physiol Actions

Reactivity with other species has not been tested.
This monoclonal antibody recognizes the brachyury protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

47 kDa
The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Immunogen

Epitope: C-terminus
Synthetic human brachyury peptide, a.a. 224-435.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chromatin remodeling during mouse and human embryonic stem cell differentiation.
Golob, Jonathan L, et al.
Developmental Dynamics, 237, 1389-1398 (2008)
Brachyury, a crucial regulator of notochordal development, is a novel biomarker for chordomas.
Vujovic, S, et al.
The Journal of Pathology, 209, 157-165 (2006)
T P Yamaguchi et al.
Genes & development, 13(24), 3185-3190 (2000-01-05)
Wnt3a encodes a signal that is expressed in the primitive streak of the gastrulating mouse embryo and is required for paraxial mesoderm development. In its absence cells adopt ectopic neural fates. Embryos lacking the T-box-containing transcription factors, Brachyury or Tbx6
Hematopoiesis from human embryonic stem cells: overcoming the immune barrier in stem cell therapies.
Priddle, Helen, et al.
Stem Cells, 24, 815-824 (2006)
Development of antibodies to human embryonic stem cell antigens.
Cai, Jingli, et al.
BMC Developmental Biology, 5, 26-26 (2005)
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