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04-1530

Anti-MDM2 Antibody, clone 3G9

Name: Anti-MDM2 Antibody, clone 3G9
Brand: MM

clone 3G9, from mouse

Synonym(s):

Double minute 2 protein, Mdm2 p53 binding protein homolog (mouse), Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), Oncoprotein Mdm2, double minute 2, human homolog

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

3G9, monoclonal

Application:

ICC, IHC, IP, WB

Citations:

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Properties

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3G9, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

NCBI accession no.

NP_002383.2

UniProt accession no.

Q00987

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... MDM2(4193)
mouse ... Mdm2(17246)

Description

General description

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

~ 75 kDa

Immunogen

Epitope: Unknown

His-tagged recombinant protein corresponding to human MDM2.

Application

Anti-MDM2 Antibody, clone 3G9 is a Mouse Monoclonal Antibody for detection of MDM2 also known as Double minute 2 protein or Mdm2 p53 binding protein homolog & has been validated in WB, IP, ICC & IHC.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Ubiquitin & Ubiquitin Metabolism

Biochem/physiol Actions

This antibody recognizes MDM2.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF-7 cell lysate

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM2 on 10 µg of MCF-7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c