Anti-GluR1 Antibody, clone C3T, rabbit monoclonal

L glutamate is the major excitatory neurotransmitter in the central nervous system. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors that have been divided into 3 groups. Group I includes GRM1 and these receptors have been shown to activate phospholipase C. GRM1 expression has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries.

Predicted to cross-react with Chimpanzee based pm 100% sequence homology.

Recognizes GluR1, Mr 106 kDa.

KLH-conjugated, synthetic peptide corresponding to amino acids 858-868 (..TLPRNSGAGAS..) of human GluR1 (amino acids 840-850 of mature human GluR1)

Anti-GluR1 Antibody, clone C3T detects level of GluR1 & has been published & validated for use in IH, IP & WB.

Immunoprecipitation: 2 μL of a previous lot immunoprecipitated GluR1 from rat brain microsomal preparation.

Immunohistochemistry: A previous lot of this antibody has been shown to detect GluR1 in human, mouse and rat tissues.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Routinely evaluated by Western blotting of rat brain microsomal preparation.

Western Blotting Analysis (WB): A 1:5,000-1:10,000 dilution of this lot detected GluR1 in rat brain microsomal preparation (Catalog # 12-144).

106 kDa

Replaces: 05-855

Cultured supernantant in 0.05% sodium azide

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Routinely evaluated by immunoblot on rat brain microsomal preparation (12-144)

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