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04-858

Sigma-Aldrich

Anti-Histone H4 Antibody, pan, rabbit monoclonal

culture supernatant, clone 62-141-13, Upstate®

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Synonym(s):
H4, Histone H4
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

62-141-13, monoclonal

species reactivity

Saccharomyces cerevisiae, human, vertebrates, chicken

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003538.3

UniProt accession no.

P62805

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... H4C1(8359)

General description

This antibody recognizes histone H4 independant of any post-translational modifications.

Specificity

Recognizes Histone H4.

Immunogen

Epitope: a.a. 25-28
Synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28

Application

Anti-Histone H4 Antibody, pan is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex.
Immunoblot Analysis:
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).

Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Routinely evaluated by immunoblot using HeLa cell acid extract.

Target description

10 kDa

Linkage

Replaces: 05-858

Physical form

Cultured supernantant in 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.

Legal Information

Luminex is a registered trademark of Luminex Corp
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Multiple forms of H4 histone mRNA in human cells.
Lichtler, A C, et al.
Proceedings of the National Academy of Sciences of the USA, 77, 1942-1946 (1980)
Yuzhi Wang et al.
Breast cancer research : BCR, 20(1), 83-83 (2018-08-04)
Although numerous studies have reported that tricho-rhino-phalangeal syndrome type I (TRPS1) protein, the only reported atypical GATA transcription factor, is overexpressed in various carcinomas, the underlying mechanism(s) by which it contributes to cancer remain unknown. Both overexpression and knockdown of
Mark M Painter et al.
Journal of virology, 91(24) (2017-10-13)
Human immunodeficiency virus type 1 (HIV-1) establishes transcriptionally silent latent infections in resting memory T cells and hematopoietic stem and progenitor cells (HSPCs), which allows the virus to persist in infected individuals despite antiretroviral therapy. Developing in vitro models of
Deepika Jaiswal et al.
G3 (Bethesda, Md.), 7(12), 3971-3982 (2017-10-27)
The conserved yeast histone methyltransferase Set1 targets H3 lysine 4 (H3K4) for mono, di, and trimethylation and is linked to active transcription due to the euchromatic distribution of these methyl marks and the recruitment of Set1 during transcription. However, loss
Chun-Song Yang et al.
Molecular cell, 66(4), 503-516 (2017-05-20)
ADP-ribosylation of proteins is emerging as an important regulatory mechanism. Depending on the family member, ADP-ribosyltransferases either conjugate a single ADP-ribose to a target or generate ADP-ribose chains. Here we characterize Parp9, a mono-ADP-ribosyltransferase reported to be enzymatically inactive. Parp9
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