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Merck
CN

05-1049

Anti-Met (extracellular) Antibody, clone 4F8.2

clone 4F8.2, from mouse

Synonym(s):

HGF receptor, HGF/SF receptor, Met proto-oncogene tyrosine kinase, SF receptor, Scatter factor receptor, met proto-oncogene, met proto-oncogene (hepatocyte growth factor receptor), oncogene MET

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4F8.2, monoclonal
Application:
immunohistochemistry
western blot
Species reactivity:
human
Citations:
5
Technique(s):
immunohistochemistry: suitable (paraffin)
western blot: suitable
Uniprot accession no.:
P08581

Key Documents

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Product Name

Anti-Met (extracellular) Antibody, clone 4F8.2, clone 4F8.2, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

4F8.2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NP_001120972.1

UniProt accession no.

P08581

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... MET(4233)

Analysis Note

Routinely evaluated by Western Blot.

Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.

Application

Detect Met (extracellular) using this Anti-Met (extracellular) Antibody, clone 4F8.2 validated for use in IH(P) & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors
Western Blot Analysis:
This lot detected Met at 1:1,000 dilution in HEK293 cell lysate resolved via SDS-PAGE and transferred to PVDF.

Immunohistochemistry Analysis :

Biochem/physiol Actions

Detects Met on its extracellular domain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

170 kDa
The proto-oncogene Met (c-Met, HGF/SF Receptor, HGFR) is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. This stimulates the autophosphorylation on Tyr1234/Tyr1235 which leads to pleiotropic downstream signaling events in epithelial cells that stimulate multiple cellular processes including cell proliferation, growth, differentiation, cell migration/ invasion, and tumorigenesis. These effects are mediated by SH2 domain-containing proteins that bind to the C-terminal tail of c-Met. Chronic stimulation of Met on cancer cells is thought to play a role in metastasis and dysregulation of c-Met activity is directly associated with oncogenesis, making it an important cancer target.

Immunogen

Epitope: Extracelluar domain (N-term)
GST-fusion protein corresponding to amino acids 80-434 of human Met.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 05-237

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ilia Atanelishvili et al.
PloS one, 11(9), e0162357-e0162357 (2016-09-02)
Pulmonary fibrosis represents the terminal stage of a diverse group of lung diseases including scleroderma associated interstitial lung disease. The molecular mechanisms underlying the pathogenesis of lung fibrosis are not well understood and there is a great need for more
Charles Finsterwald et al.
The European journal of neuroscience, 34(7), 1053-1061 (2011-09-08)
Acquisition of a mature dendritic morphology is critical for neural information processing. In particular, hepatocyte growth factor (HGF) controls dendritic arborization during brain development. However, the cellular mechanisms underlying the effects of HGF on dendritic growth remain elusive. Here, we
Christopher C Phelps et al.
Infection and immunity, 86(10) (2018-08-01)
Listeria monocytogenes is a facultative intracellular pathogen that infects a wide variety of cells, causing the life-threatening disease listeriosis. L. monocytogenes virulence factors include two surface invasins, InlA and InlB, known to promote bacterial uptake by host cells, and the
Nolan L Boyd et al.
Tissue engineering. Part A, 19(1-2), 211-223 (2012-09-14)
The microvasculature is principally composed of two cell types: endothelium and mural support cells. Multiple sources are available for human endothelial cells (ECs) but sources for human microvascular mural cells (MCs) are limited. We derived multipotent mesenchymal progenitor cells from

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