VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines.

Detects the 121, 165, and 189 amino acid isoforms of VEGF.

Recombinant human VEGF 189aa isoform

Detect VEGF using this Anti-VEGF Antibody, clone VG1 validated for use in WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected VEGF on 10 µg of NIH/3T3 cell lysate.

Approx. 12-28 kDa

Replaces: MAB3734

Unpurified

Unpurified mouse monoclonal IgG1κ in ascites fluid containing no preservatives.

Stable for 12 months at -20°C from date of receipt.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
NIH/3T3 cell lysate

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