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Merck
CN

05-1248

Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306

clone CMA306, from mouse

Synonym(s):

H3K9me1, Histone H3 (mono methyl K9), H3-K9-HMTase 1, Histone H3-K9 methyltransferase 1, Lysine N-methyltransferase 1A, Position-effect variegation 3-9 homolog, Su(var)3-9 homolog 1, Suppressor of variegation 3-9 homolog 1, histone-lysine N-methyltransfe

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306, clone CMA306, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CMA306, monoclonal

species reactivity

human

technique(s)

inhibition assay: suitable (peptide)
western blot: suitable

isotype

IgG2a

NCBI accession no.

NP_003484

UniProt accession no.

O43463

shipped in

wet ice

target post-translational modification

monomethylation (Lys9)

Quality Level

100

Gene Information

human ... H3C1(8350)

Analysis Note

Control
Recombinant Histone H3 and HeLa acid extract.
Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.

Application

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.

Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 (Mouse Monoclonal Antibody) validated in WB, PIA to detect monomethyl Histone H3 (Lys9) also known as H3K9me1.

Biochem/physiol Actions

This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
~17 kDa

Immunogen

Epitope: monomethylated H3 Lys9
KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.

Preparation Note

Stable at 2-8°C for 1 year from date of receipt.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The Journal of Biological Chemistry null
Jing-Ru Hao et al.
Basic & clinical pharmacology & toxicology, 132(2), 180-196 (2022-11-03)
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