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Sigma-Aldrich

Anti-EED Antibody, clone AA19

ascites fluid, from mouse

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Synonym(s):
WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

monoclonal

species reactivity

human, mouse

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_068676

UniProt accession no.

O75530

target post-translational modification

unmodified

Gene Information

human ... EED(8726)
mouse ... Eed(13626)

General description

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. EED is also known to bind H3K27me3 and can allosterically stimulate the methyltransferase activity of the PRC2 complex. (Ref.: Hojfeldt, JW. et al (2018) Nat. Struc. Mol. Biol. 25 (3); 225-232).

Specificity

Not tested in other species.
This antibody recognizes mouse EED, (~30-65 kDa).

Immunogen

Recombinant fusion protein of full length murine EED tagged with MBP.

Application

Anti-EED Antibody, clone AA19 is a mouse monoclonal antibody for detection of EED also known as WD protein associating with integrin cytoplasmic tails 1 ,embryonic ectoderm development & has been validated in WB.
Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer
Research Sub Category
Chromatin Biology

Quality

Routinely evaluated by Western Blot on A431cel lysatel.

Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.

Target description

Ranges from 30-65 kDa (5-6 isoforms of EED are detected).

Physical form

Protein G Purified
Purified immunoglobulin in 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
A431 Cell Lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Polycomb group proteins in cell cycle progression and cancer.
Pasini, Diego, et al.
Cell Cycle, 3, 396-400 (2004)
The central role of EED in the orchestration of polycomb group complexes.
Cao, Q; Wang, X; Zhao, M; Yang, R; Malik, R; Qiao, Y; Poliakov, A; Yocum, AK; Li, Y; Chen et al.
Nature Communications null
Adrian P Bracken et al.
Genes & development, 20(9), 1123-1136 (2006-04-19)
The Polycomb group (PcG) proteins form chromatin-modifying complexes that are essential for embryonic development and stem cell renewal and are commonly deregulated in cancer. Here, we identify their target genes using genome-wide location analysis in human embryonic fibroblasts. We find
Adrian P Bracken et al.
The EMBO journal, 22(20), 5323-5335 (2003-10-09)
Recent experiments have demonstrated that the Polycomb group (PcG) gene EZH2 is highly expressed in metastatic prostate cancer and in lymphomas. EZH2 is a component of the PRC2 histone methyltransferase complex, which also contains EED and SUZ12 and is required
James P Reddington et al.
Genome biology, 14(3), R25-R25 (2013-03-28)
DNA methylation and the Polycomb repression system are epigenetic mechanisms that play important roles in maintaining transcriptional repression. Recent evidence suggests that DNA methylation can attenuate the binding of Polycomb protein components to chromatin and thus plays a role in
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