Anti-hnRNP Q Antibody, clone 18E4

Heterogeneous nuclear ribonucleoprotein Q commoly refered to as hnRNP Q can be found as three different isoforms derived from alternative splicing of a single gene. This RNA binding protein is implicated in control of mRNA precursor splicing for the survival of motor neurons (SMN) protein, a gene. Loss-of-function mutations of SMN are linked to spinal muscular atrophy (SMA) a common neurodegenerative disease. It has been shown that hnRNP Q proteins interact with SMN, and this is interacton is required for efficient pre-mRNA splicing in vitro. Current data suggests that hnRNP Q is a splicing modulator of SMN.

This antibody recognizes hnRNP Q.

Epitope: Unknown

Recombinant protein corresponding to human hnRNP Q.

Immunocytochemistry
Cited by independent researcher using a representative lot.
Immunoprecipitation
Cited by independent researcher using a representative lot.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-hnRNP Q Antibody, clone 18E4 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect hnRNP Q also known as heterogeneous nuclear ribonucleoprotein Q-like.

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected hnRNP Q on 10 µg of HeLa cell lysate.

The calculated molecular weight is 70 kDa Clone 18E4 can recognizes four bands on the Western Blot of total HeLa cell lysate corresponding to molecular masses of ~80, ~70, ~60 and ~55 kDa hnRNP Q has three isoforms of ~70– 55 kDa The ~80 kDa reactive band was identified as hnRNP R, which migrates at ~80 kDa on SDS–PAGE. (Mourelatos., Z., et al., EMBO J., 20, 5443-5452 (2001).)

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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