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Sigma-Aldrich

Endothelin 1, Human and Porcine

Endothelin 1, Human and Porcine, CAS 117399-94-7, is a 21-amino acid polypeptide with potent vasoconstrictive action. Activates phospholipase C in fibroblasts expressing ETA receptors.

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Synonym(s):
Endothelin 1, Human and Porcine, ET-1, ET-1, CSCSSLMDKECVYFCHLDIIW, PPET1, Preproendothelin-1
Empirical Formula (Hill Notation):
C109H159N25O32S5
CAS Number:
117399-94-7
Molecular Weight:
2491.90
MDL number:
MFCD00133305

biological source

Porcine
human

Quality Level

100

Assay

≥96% (HPLC)

form

solid

mol wt

2491.9 g/mol

manufacturer/tradename

Calbiochem®

storage condition

(Tightly closed Dry)
OK to freeze
desiccated

technique(s)

cell culture | mammalian: suitable

color

white to off-white

solubility

5% acetic acid: 0.5 mg/mL

shipped in

ambient

storage temp.

−20°C

InChI

1S/C109H159N25O32S5/c1-12-56(9)87(107(163)125-76(109(165)166)39-60-43-113-65-24-18-17-23-63(60)65)134-108(164)88(57(10)13-2)133-99(155)75(42-85(143)144)123-94(150)70(36-54(5)6)118-97(153)73(40-61-44-112-52-114-61)121-103(159)80-49-169-168-48-64(111)89(145)126-77(45-135)102(158)131-81-50-170-171-51-82(105(161)132-86(55(7)8)106(162)124-72(38-59-26-28-62(138)29-27-59)95(151)120-71(96(152)130-80)37-58-21-15-14-16-22-58)129-91(147)67(30-31-83(139)140)116-90(146)66(25-19-20-33-110)115-98(154)74(41-84(141)142)122-92(148)68(32-34-167-11)117-93(149)69(35-53(3)4)119-100(156)78(46-136)127-101(157)79(47-137)128-104(81)160/h14-18,21-24,26-29,43-44,52-57,64,66-82,86-88,113,135-138H,12-13,19-20,25,30-42,45-51,110-111H2,1-11H3,(H,112,114)(H,115,154)(H,116,146)(H,117,149)(H,118,153)(H,119,156)(H,120,151)(H,121,159)(H,122,148)(H,123,150)(H,124,162)(H,125,163)(H,126,145)(H,127,157)(H,128,160)(H,129,147)(H,130,152)(H,131,158)(H,132,161)(H,133,155)(H,134,164)(H,139,140)(H,141,142)(H,143,144)(H,165,166)

InChI key

ZUBDGKVDJUIMQQ-UHFFFAOYSA-N

General description

Research area: Cell Signaling

Endothelin 1 (ET-1) is a peptide hormone located in the endothelial cells and belongs to the endothelin family. It is a derivative of pre-pro-ET-1 which is later converted to mature ET-1 by a group of ET-converting enzymes (ECEs), endopeptidases, chymases, and non-ECE metalloproteinases. The EDN1 gene is mapped to the human chromosome at 6p24.1.

Application

Endothelin 1, Human and Porcine - CAS 117399-94-7 – Calbiochem has been used:
  • to study its effects on the production of erythropoietin under normoxia and carbon monoxide-induced hypoxia in rat models
  • as a protein kinase C (PKC) agonist to study its effects on the phosphorylation of myofilament proteins in rat myocytes
  • to induce a hypertrophic-like state to study its effects on contraction pressure in normal and mutated human induced pluripotent stem cells (hiPSC)-derived cardiomyocytes

Biochem/physiol Actions

Endothelin 1 (ET-1) acts as a mediator of paracrine and autocrine functions. The production of ET-1 is upregulated under pathophysiological conditions and is produced in several types of cells such as cardiac myocytes, vascular smooth muscle cells, and inflammatory cells like leukocytes and macrophages. ET-1 is a potent vasoconstrictor peptide that is stimulated by risk factors in several types of cells for cardiovascular diseases. It is involved in many biological effects such as pro-inflammatory actions, platelet activation, stimulation of free radicals, and mitogenic and proliferative effects. ET-1 plays a role in pulmonary physiology, neurological function, autoimmune disorders, transportation of electrolytes and fluids, and cancer. It is an essential component for the development of embryonic neural crest-derived tissues. ET-1 is involved in osteogenesis by promoting osteoblast proliferation by activating Wnt signaling pathway.

Packaging

Yes

Warning

Toxicity: Standard Handling (A)

Sequence

H-Cys¹-Ser-Cys³-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys¹¹-Val-Tyr-Phe-Cys¹⁵-His-Leu-Asp-Ile-Ile-Trp-OH (disulfide bonds: 1 → 15; 3 → 11)

Physical form

Supplied as an acetate salt.

Preparation Note

Dissolve peptide initially at 8 mg/ml in 80% cold acetic acid, then dilute with distilled water to achieve 5% acetic acid and 0.5 mg/ml peptide.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 weeks at -20°C.

Other Notes

Cesari, M., et al. 1996. Am. Heart J.132, 1236.
Gresser, O., et al 1996. Biochem. Biophys. Res. Commun.224, 169.
Murata, S., et al. 1995. J. Pharmacol. Exp. Ther. 274, 1524.
Hexum, T.D., et al. 1990. Biochem. Biophys. Res. Commun. 167, 294.
Itoh, Y., et al. 1988. FEBS Lett. 231, 440.
Yanagisawa, M., et al. 1988. Nature 332, 411.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

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Indranil Chattopadhyay et al.
Mutation research, 696(2), 130-138 (2010-01-20)
Genomic alterations such as chromosomal amplifications, deletions and loss of heterozygosity play an important role in the pathogenesis and progression of cancer. Environmental risk factors contribute to the development and progression of tumors by facilitating the loss of tumor suppressor
A Grenz et al.
Naunyn-Schmiedeberg's archives of pharmacology, 373(5), 342-348 (2006-08-10)
It has been hypothesized that autacoids, such as endothelin-1 (ET), may modulate erythropoietin (Epo) secretion. Therefore, we studied the effect of ET-1 infusion and of a nonselective ET(A/B) receptor antagonist on Epo secretion under carbon monoxide (CO) exposure. Anesthetized rats
Gregory A Clines et al.
Molecular endocrinology (Baltimore, Md.), 21(2), 486-498 (2006-10-28)
Tumor-produced endothelin-1 (ET-1) stimulates osteoblasts to form new bone and is an important mediator of osteoblastic bone metastasis. The anabolic actions of ET-1 in osteoblasts were investigated by gene microarray analyses of murine neonatal calvarial organ cultures. Targets of ET-1
Lisa R Stow et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(1), 16-28 (2010-09-15)
Over two decades of research have demonstrated that the peptide hormone endothelin-1 (ET-1) plays multiple, complex roles in cardiovascular, neural, pulmonary, reproductive, and renal physiology. Differential and tissue-specific production of ET-1 must be tightly regulated in order to preserve these
Felix Böhm et al.
Cardiovascular research, 76(1), 8-18 (2007-07-10)
Endothelin (ET)-1 is a potent vasoconstrictor peptide originally isolated from endothelial cells. Its production is stimulated in a variety of different cell types under the influence of risk factors for cardiovascular disease and during the development of cardiovascular disease. Based
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