05-372
Anti-GRB2 Antibody, clone 3F2
clone 3F2, Upstate®, from mouse
别名:
Anti-ASH, Anti-EGFRBP-GRB2, Anti-Grb3-3, Anti-MST084, Anti-MSTP084, Anti-NCKAP2
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3F2, monoclonal
Application:
IHC, WB
Citations:
23
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3F2, monoclonal
species reactivity
bovine, mouse, hamster, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GRB2(2885)
General description
23-25kDa
Immunogen
Recombinant GRB2/Ash
Application
This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.
Biochem/physiol Actions
N-terminal SH3 region of GRB2
Physical form
Format: Purified
PBS containing 50% glycerol
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable