05-372
Anti-GRB2 Antibody, clone 3F2
clone 3F2, Upstate®, from mouse
别名:
Anti-ASH, Anti-EGFRBP-GRB2, Anti-Grb3-3, Anti-MST084, Anti-MSTP084, Anti-NCKAP2
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3F2, monoclonal
Application:
IHC, WB
Citations:
23
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3F2, monoclonal
species reactivity
bovine, mouse, hamster, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GRB2(2885)
General description
23-25kDa
Immunogen
Recombinant GRB2/Ash
Application
This Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.
Biochem/physiol Actions
N-terminal SH3 region of GRB2
Physical form
Format: Purified
PBS containing 50% glycerol
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Protein-tyrosine phosphatase 1D modulates its own state of tyrosine phosphorylation
Stein-Gerlach, M., et al
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c-Cbl is transiently tyrosine-phosphorylated, ubiquitinated, and membrane-targeted following CSF-1 stimulation of macrophages
Wang, Y., et al
The Journal of Biological Chemistry, 271, 17-20 (1996)
M Rozakis-Adcock et al.
Nature, 363(6424), 83-85 (1993-05-06)
Many tyrosine kinases, including the receptors for hormones such as epidermal growth factor (EGF), nerve growth factor and insulin, transmit intracellular signals through Ras proteins. Ligand binding to such receptors stimulates Ras guanine-nucleotide-exchange activity and increases the level of GTP-bound
M Shakibaei et al.
The Biochemical journal, 342 Pt 3, 615-623 (1999-09-08)
We have examined the mechanism by which collagen-binding integrins co-operate with insulin-like growth factor-I (IGF-I) receptors (IGF-IR) to regulate chondrocyte phenotype and differentiation. Adhesion of chondrocytes to anti-beta1 integrin antibodies or collagen type II leads to phosphorylation of cytoskeletal and
Ramya Ganesan et al.
Journal of leukocyte biology, 103(5), 867-883 (2018-04-16)
The uptake of cholesterol carried by low-density lipoprotein (LDL) is tightly controlled in the body. Macrophages are not well suited to counteract the cellular consequences of excess cholesterol leading to their transformation into "foam cells," an early step in vascular
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