Anti-CrkL Antibody, clone 5-6

39kDa

CRKL is a 39kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.
The antibody does not cross-react with CRKI or CRKII.

Epitope: Full-length

Full length GST fusion protein corresponding to Human CRKL

Detect CrkL using this Anti-CrkL Antibody, clone 5-6 validated for use in IP, WB, IH.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Other species have not been tested.

the C-terminal SH3 of Human CRKL

Format: Purified

Protein G Purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.

Control
Western Blot and Immunoprecipitation:
K562 cell lysate
Immunocytochemistry:
Hela cells

Routinely evaluated by Western Blot on K562 cell lysate.

Western Blot Analysis: Antibody detected CRKL in 15 μg of K562 cell lysate.

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