05-481
Anti-phospho-MAP Kinase1/2 Antibody
clone 12D4, Upstate®, from mouse
Synonym(s):
Extracellular signal-regulated kinase, Insulin-stimulated MAP2 kinase, MAP kinase 1, MAPK 1, Microtubule-associated protein 2 kinase, extracellular signal-related kinase 1, mitogen-activated protein kinase 3
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
12D4, monoclonal
Species reactivity:
avian, rat, mouse, canine, human
Application:
ELISA
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
Technique(s):
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Citations:
36
Uniprot accession no.:
Product Name
Anti-phospho-MAP Kinase1/2 Antibody, clone 12D4, Upstate®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12D4, monoclonal
species reactivity
avian, rat, mouse, canine, human
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr202/pTyr204)
Quality Level
Gene Information
human ... MAPK1(5594)
Analysis Note
Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Evaluated by western blot on RIPA lysates from EGF-stimulated human A431 carcinoma cells.
Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells.
Western Blot Analysis: 0.1-1.0 μg/mL of this antibody detected phospho-MAPK in RIPA lysates from EGFstimulated human A431 carcinoma cells.
Application
Detect phospho-MAP Kinase1/2 using this Anti-phospho-MAP Kinase1/2 validated for use in WB, IP, ELISA & IC.
Immunoprecipitation: Use 1-10 μg per immuno-precipitation.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Biochem/physiol Actions
Specific for phosphorylated (activated) Erk1 and Erk2. Antibody recognition requires phosphorylation of both the threonine and tyrosine residues of Erk1/Erk2 and will not interact with the non-phosphorylated form of the proteins. Does not cross react with activated SAP Kinase 1 or 2.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
42-44 kDa
Mitogen-activated protein kinase 3 (UniProt: P27632; also known as EC:2.7.11.24, MAP kinase 3, MAPK 3. ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase p44-ERK1) is encoded by the MAPK3 (also known as ERK1, PRKM3) gene (Gene ID: 5595) in human. ERK-1 is a serine/threonine kinase that acts as an essential component of the MAP kinase signal transduction pathway. It is expressed in different tissues and its highest levels have been observed in the nervous system. The MAPK/ERK cascade mediates several diverse biological processes such as cell growth, adhesion, survival, and differentiation through the regulation of transcription, translation, and cytoskeletal rearrangements. It also plays a role in initiation and regulation of meiosis, mitosis, and post-mitotic functions in differentiated cells by phosphorylating a few transcription factors. About 160 substrates have been described for ERKs. The protein kinase domain of ERK-1 is localized to amino acids 42-330 and the TXY motif is located in amino acids 202-204. ERK-1 is phosphorylated by MEK1 and MEK2 on threonine 202 and tyrosine 204 in response to external stimuli like insulin or nerve growth factor and both these phosphorylations are essential for its activity. Three isoforms of ERK-1 have been described that are produced by alternative splicing.
Immunogen
KLH-conjugated phosphopeptide containing the pTEpY motif of Erk1 and Erk2. Clone: 12D4
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified mouse monoclonal IgG1k lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose.
Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL.
Reconstitute with 1 mL H2O (15 min, RT) for a final concentration of 100 μg/mL.
Thiophilic adsoption and size exclusion chromatography
Preparation Note
Stable for 1 year at from date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Certificates of Analysis (COA)
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Julia Yuen-Shan Tsang et al.
Journal of leukocyte biology, 80(1), 145-151 (2006-05-11)
CD4+CD25+ regulatory T cells play an important role in peripheral tolerance. Upon T cell receptor (TCR)-mediated activation, the cells fail to proliferate but are induced to have a suppressor function. The intracellular signaling events that lead to their responses have
Yingzi Chang et al.
Current research in pharmacology and drug discovery, 3, 100094-100094 (2022-03-19)
Basic fibroblast growth factor (bFGF)-mediated vascular smooth muscle cell (VSMC) proliferation and migration play an important role in vascular injury-induced neointima formation and subsequent vascular restenosis, a major event that hinders the long-term success of angioplasty. The function of β3-adrenergic
Diversity in function and regulation of MAP kinase pathways.
Blumer, K J and Johnson, G L
Trends in Biochemical Sciences, 19, 236-240 (1994)
Nerve growth factor stimulates a novel protein kinase in PC-12 cells that phosphorylates and activates mitogen-activated protein kinase kinase (MEK).
Pang, L, et al.
The Biochemical Journal, 307 ( Pt 2), 513-519 (1995)
Hyaluronan and the interaction between CD44 and epidermal growth factor receptor in oncogenic signaling and chemotherapy resistance in head and neck cancer.
Wang, SJ; Bourguignon, LY
Archives of Otolaryngology--Head & Neck Surgery null
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