05-531
Anti-His Tag Antibody
clone 4D11, mouse monoclonal, Upstate
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Clone:
4D11, monoclonal
Technique(s):
immunocytochemistry: suitable, western blot: suitable
Application:
ICC, WB
Citations:
13
Product Name
Anti-Histidine Tagged Antibody, clone 4D11, clone 4D11, Upstate®, from mouse
biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
4D11, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG2aκ
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HRG(3273)
General description
The apparent molecular weight is related to the tagged protein.
Immunogen
N-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.
Application
This Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.
Biochem/physiol Actions
Recognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag
Physical form
Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.
Analysis Note
Control
Cells expressing the His-tag
Cells expressing the His-tag
routinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ruiping Zheng et al.
Journal of cell science, 123(Pt 21), 3734-3744 (2010-10-14)
In higher eukaryotic cells, long non-protein-coding RNAs (lncRNAs) have been implicated in a wide array of cellular functions. Cell- or tissue-specific expression of lncRNA genes encoded in the mammalian genome is thought to contribute to the complex gene networks needed
Lisa A Mitchell et al.
Molecular human reproduction, 14(4), 235-243 (2008-02-05)
The process of capacitation is a pre-requisite for mammalian spermatozoa allowing them to gain the ability to fertilize an oocyte. A fundamental part of this mechanism is a dramatic increase in the level of tyrosine phosphorylation. Implicated in this process
Wei-Hang Huang et al.
Heliyon, 9(11), e21486-e21486 (2023-11-29)
Originally extracted from Momordica charantia seeds, the antiviral and anti-tumor activities of Momordica anti-HIV protein MAP30 have become well known. Although MAP30 has been reported to possess antiviral activity against several human viruses, the current understanding of the MAP30-mediated antiviral