05-594
Anti-HSP90 Antibody
clone D7a, Upstate®, from mouse
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
D7a, monoclonal
Application:
IHC, IP, WB
Citations:
13
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
D7a, monoclonal
species reactivity
human, rabbit, pig, mouse, rat, bovine, chicken
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... HSP90AA1(3320)
General description
90 kDa
Immunogen
Full-length protein purified from chicken brain; clone D7a
Application
This Anti-HSP90 Antibody is validated for use in IP, WB for the detection of HSP90.
Biochem/physiol Actions
Recognizes HSP90.
Physical form
Format: Purified
Protein G Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts or human A431 cells
Other Notes
Please refer to lot specific datasheet.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
wgk
WGK 1
Regulatory Information
常规特殊物品
低风险生物材料
This item has
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Jason M Shapiro et al.
PloS one, 11(11), e0165227-e0165227 (2016-11-02)
We previously isolated AF20, a murine monoclonal antibody that recognizes a cell surface glycoprotein of approximately 90-110 kDa. The AF20 antigen is specifically expressed in human hepatoma and colon cancer cell lines, and thus could serve as a cancer biomarker.
The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors.
Pratt, W B
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 217, 420-434 (1998)
Miquéias Lopes-Pacheco et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(6), 2194-2210 (2017-04-28)
Premature degradation of mutated cystic fibrosis transmembrane conductance regulator (CFTR) protein causes cystic fibrosis (CF), the commonest Mendelian disease in Caucasians. Despite recent advances in precision medicines for CF patients, many CFTR mutants have not been characterized and the effects
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-594-25UG | 04054839350207 |