05-677
Anti-phospho-Src (Tyr416) Antibody, clone 9A6
clone 9A6, Upstate®, from mouse
Synonym(s):
proto-oncogene tyrosine-protein kinase SRC, protooncogene SRC, Rous sarcoma, tyrosine kinase pp60c-src, tyrosine-protein kinase SRC-1, v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homo
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
9A6, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG3
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr416)
Gene Information
human ... SRC(6714)
General description
60 kDa
Specific src isoform is unknown
Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated.
Immunogen
KLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src. Clone 9A6.
Application
Detect phospho-Src (Tyr416) using this Anti-phospho-Src (Tyr416) Antibody, clone 9A6 validated for use in WB & IP.
Immunoprecipitation/Immunoblot Analysis: 4 μg/mL of a previous lot detected phospho-Src immunoprecipitated from 3T3 cell lysates with 10 μg of anti-Src (Catalog # 05-184), but not after phosphatase treatment with YOP.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Recognizes phospho-Src, Mr 60kDa. The antibody does not crossreact with the nonphosphorylated form of Src or with unrelated phosphorylation sites. Predicted to recognize all Src family members phosphorylated at the tyrosine corresponding to Tyr416 of avian Src.
Physical form
Format: Purified
Purified mouse monoclonal IgG3 lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 mL H2O (15 min, RT). Lyophilized. Store at -20ºC.
Thiophilic adsoption and size exclusion chromatography
Preparation Note
Stable for 1 year at from date of receipt.
Analysis Note
Control
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
Evaluated by immunoprecipitating Src from 3T3 cell lysates with anti-Src (Catalog #05-184), but not after phosphatase treatment with YOP.
Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.
Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Edwin K Jackson et al.
The Journal of pharmacology and experimental therapeutics, 314(3), 1109-1116 (2005-05-20)
alpha(2)-Adrenoceptors potentiate vascular responses to angiotensin II. The goal of this study was to test the hypothesis that the phospholipase C (PLC)/protein kinase C (PKC)/c-src/phosphatidylinositol 3-kinase (PI3K) pathway contributes to the vascular angiotensin II/alpha(2)-adrenoceptor interaction. In rats in vivo, intrarenal
K Bhaskar et al.
Neuropathology and applied neurobiology, 36(6), 462-477 (2010-07-09)
Tau protein is a prominent component of paired helical filaments in Alzheimer's disease (AD) and other tauopathies. While the abnormal phosphorylation of tau on serine and threonine has been well established in the disease process, its phosphorylation on tyrosine has
A Sirvent et al.
Oncogene, 29(9), 1303-1315 (2009-12-17)
The nonreceptor tyrosine kinases of the Src family (SFK) are frequently deregulated in human colorectal cancer (CRC), and they have been implicated in tumour growth and metastasis. How SFK are activated in this cancer has not been clearly established. Here
K Miekus et al.
Cell death & disease, 4, e459-e459 (2013-01-19)
Rhabdomyosarcoma (RMS) is the most common type of pediatric soft tissue sarcoma. The MET receptor has an important role in the biology of RMS, and its overexpression and hyperactivation correlate with the metastatic ability of RMS. Consequently, interfering with MET
T Koreckij et al.
British journal of cancer, 101(2), 263-268 (2009-07-16)
Dasatinib is a small molecule kinase inhibitor that has recently been shown to inhibit Src family kinases (SFK) and also has activity against CaP. Of importance to metastatic CaP, which frequently metastasises to bone, SFK are also vital to the
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