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Sigma-Aldrich

Anti-Myc Tag Antibody, clone 4A6

clone 4A6, Upstate®, from mouse

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Synonym(s):
Anti-CMyc
eCl@ss:
32160702

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4A6, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002467.3

UniProt accession no.

P01106

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)

General description

Identification of the target epitope is important for characterization of a monoclonal antibodies. Epitope tags are short, linear antibody recognition sequences that enable detection of tagged fusion proteins by antibodies. Over the years, a wide range of anti-tag antibodies have been developed and each one of these offer unique advantages and disadvantages. Epitope tags can undergo post-translational modifications, such as phosphorylation and glycosylation in cells and cause an increase in the molecular mass of the fused protein, which can result in changes in gel mobility. Epitope tags of smaller number of amino acid residues are advantageous in that they exhibit minimal side effects on structure and function of fused target protein. A Myc tag can be used in many different immunoassays and is useful when there is no antibody against the protein of interest. Adding a Myc-tag permits the detection of such proteins with an antibody against the Myc epitope. Clone 4A6 is an antibody that recognizes ten amino acid sequence EQKLISEEDL (Glu-Gln-Lys-Leu-Iso-Ser-Glu-Glu-Asp-Leu) corresponding to the C-terminal region of human c-Myc protein (UniProt: P01106). Unlike other clones whose performance may be affected when Myc tag sequence is followed by smaller neutral amino acids, Clone 4A6 offers much more consistent detection of Myc-tagged proteins irrespective of any changes in neighboring amino acid sequences. (Ref. Schuchner, S., et al. (2020). Sci. Signal. 13(616); eaax9730; Charest-Morin, X., et al. (2013). Front. Pharmacol. 4: 122).

Specificity

Recognizes recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts; also recognizes human Myc.

Immunogen

peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

Application

Anti-Myc Tag, clone 4A6, is a mouse monoclonal antibody that is validated for use in ChIP, IC, IF, IP and WB for the detection of Myc Tag and does not show the very high context sensitivity of 9E10. This antibody has been cited in over 480 publications.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

Quality

routinely evaluated by immunoblot on Myc-tagged recombinant protein in sequence contexts not well recognized by anti-Myc Tag, clone 9E10 (Catalogue No. CBL430)

Target description

Predicted MW for human Myc: 49 kDa

Physical form

Format: Purified
Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
Protein G Purified

Storage and Stability

Maintain at 2 to 8°C for 1 year from date of shipment.

Analysis Note

Control
Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Arabidopsis CRY2 and ZTL mediate blue-light regulation of the transcription factor CIB1 by distinct mechanisms.
Liu, H; Wang, Q; Liu, Y; Zhao, X; Imaizumi, T; Somers, DE; Tobin, EM; Lin, C
Proceedings of the National Academy of Sciences of the USA null
Identification and characterization of a novel retinoic acid response element in zebrafish cyp26a1 promoter.
Li, J; Hu, P; Li, K; Zhao, Q
Anatomical Record (Hoboken, N.J. : 2007) null
Tara M Stanne et al.
Eukaryotic cell, 10(7), 964-976 (2011-05-17)
The unicellular eukaryote Trypanosoma brucei is unusual in having very little transcriptional control. The bulk of the T. brucei genome is constitutively transcribed by RNA polymerase II (Pol II) as extensive polycistronic transcription units. Exceptions to this rule include several
Shikhar Sharma et al.
Epigenetics & chromatin, 5(1), 3-3 (2012-01-31)
DNA methylation, histone modifications and nucleosome occupancy act in concert for regulation of gene expression patterns in mammalian cells. Recently, G9a, a H3K9 methyltransferase, has been shown to play a role in establishment of DNA methylation at embryonic gene targets
Chimwar Wanglar et al.
PloS one, 9(9), e107928-e107928 (2014-09-27)
Somitogenesis in vertebrates is a complex and dynamic process involving many sequences of events generated from the segmentation clock. Previous studies with mouse embryos revealed that the presumptive somite boundary is periodically created at the anterior border of the expression
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