05-840
Anti-IDO (Indoleamine 2,3-Dioxygenase) Antibody, clone 10.1
clone 10.1, Upstate®, from mouse
Synonym(s):
Indoleamine 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase, indole 2,3-dioxygenase, indoleamine-pyrrole 2,3 dioxygenase, IDO1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
10.1, monoclonal
Species reactivity:
human, mouse
Application:
ICC, IHC, WB
Citations:
26
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10.1, monoclonal
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Gene Information
human ... IDO1(3620)
mouse ... Ido1(15930)
General description
Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
42 kDa
Immunogen
GST fusion protein corresponding to residues 78-184 of human Indoleamine 2,3- Dioxygenase (IDO). Clone 10.1.
Application
Anti-IDO (Indoleamine 2, 3-Dioxygenase) Antibody, clone 10.1 is an antibody against IDO (Indoleamine 2 for use in IC & WB.
Immunocytochemistry: A previous lot was used by an independent laboratory in Phoenix cells transfected with an expression vector.
Immunohistochemistry: A previous lot of this antibody was used to detect IDO in human retina, primary eye tumor and metastatic liver tumor tissue sections (Chen, P.W., et al. Experimental Eye Research 85(2007) 617-625).
Immunohistochemistry: A previous lot of this antibody was used to detect IDO in human retina, primary eye tumor and metastatic liver tumor tissue sections (Chen, P.W., et al. Experimental Eye Research 85(2007) 617-625).
Biochem/physiol Actions
Recognizes IDO (Indoleamine 2,3- Dioxygenase), Mr 42 kDa.
Physical form
Format: Purified
Purified mouse monoclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Analysis Note
Control
Human IFN-gamma stimulated HeLa cell lysates, IFN gamma stimulated human peripheral blood lymphocytes.
Human IFN-gamma stimulated HeLa cell lysates, IFN gamma stimulated human peripheral blood lymphocytes.
Routinely evaluated by Western Blot on untreated and IFNgamma treated (24 hrs) Hela lysates.
Western Blot Analysis:
1:500 dilution of this lot detected IDO on 10 ug of untreated and IFN gamma treated Hela lysates.
Western Blot Analysis:
1:500 dilution of this lot detected IDO on 10 ug of untreated and IFN gamma treated Hela lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1056
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Tryptophan and the immune response.
Moffett, John R and Namboodiri, Ma Aryan
Immunology and Cell Biology, 81, 247-265 (2003)
Uveal melanoma expression of indoleamine 2,3-deoxygenase: establishment of an immune privileged environment by tryptophan depletion.
Peter W Chen,Jessamee K Mellon,Elizabeth Mayhew,Shixuan Wang,Yu Guang He,Nick Hogan et al.
Experimental Eye Research null
Mechanism of interferon-gamma action. Characterization of indoleamine 2,3-dioxygenase in cultured human cells induced by interferon-gamma and evaluation of the enzyme-mediated tryptophan degradation in its anticellular activity.
Takikawa, O, et al.
The Journal of Biological Chemistry, 263, 2041-2048 (1988)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-840 | 04053252729461 |