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Merck
CN

06-1115

Anti-Interleukin-18 (IL-18) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

interleukin 18 (interferon-gamma-inducing factor), IL-1 gamma, IFN-gamma-inducing factor, iboctadekin, interleukin-18, Interleukin-1 gamma, Iboctadekin, Interferon gamma-inducing factor

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-Interleukin-18 (IL-18) Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), primate (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_001553

UniProt accession no.

Q14116

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... IL18(3606)

Analysis Note

Control
Human tonsil tissue lysate
Evaluated by Western Blot in human tonsil tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected IL-18 in 10 µg of human tonsil tissue lysate.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
This Anti-Interleukin-18 (IL-18) Antibody is validated for use in WB, IH(P) for the detection of Interleukin-18 (IL-18).
Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected IL-18 in 10 µg of human tonsil tissue lysate.

Immunohistochemistry Analysis: A 1:600 dilution from a previous lot detected IL-18 in normal human spleen tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IL-18 has been shown to be a pro-inflammatory cytokine that induces interferon-gamma. IL-18 has also been found to have the ability to activate nuclear factor-kappaB (NFkB), express Fas ligand, induce CC and CXC chemokines, and also has been found to increase the production of viable human immunodeficiency virus. IL-18 has been linked to syndromes displaying symptoms of chronic low-levels of inflammation. IL-18 also seems to have some control over homeostasis and behavior in the central nervous system as well as neuroinflammatory activity.
~22 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human Interleukin-18.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chang-Lun Huang et al.
Molecular medicine reports, 28(4) (2023-09-01)
Human parvovirus B19 (B19V) has been strongly associated with a variety of inflammatory disorders, such as rheumatoid arthritis (RA), inflammatory bowel disease and systemic lupus erythematosus. Non‑structural protein 1 (NS1) of B19V has been demonstrated to play essential roles in
C Baldini et al.
Journal of internal medicine, 274(5), 480-489 (2013-08-03)
Innate and adaptive immunity may contribute to gland dysfunction in patients with primary Sjögren's syndrome (pSS). The P2X7 receptor (P2X7 R)-NLRP3 inflammasome complex modulates the release of the inflammatory cytokines IL-1β and IL-18. The presence of P2X7 R in salivary
C Baldini et al.
Journal of internal medicine, 282(2), 175-186 (2017-05-16)
P2X7 receptor (P2X7R), trigger of acute inflammatory responses via the NLRP3 inflammasome, is hyperfunctioning in patients with Sjögren's syndrome (SS), where it stimulates IL-18 production. Some patients with SS develop a mucosa-associated lymphoid tissue non-Hodgkin's lymphoma (MALT-NHL). To prospectively evaluate

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