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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
technique(s)
immunocytochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10)
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
~17 kDa observed
Immunogen
Linear peptide corresponding to phospho-Histone H3 (Ser10).
Application
Anti-phospho-H3 (Ser10), Alexa Fluor™ 488 Conjugate is a purified rabbit polyclonal that detects Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 488 and validated in IC. The unconjugated version of this antibody is highly published and proven in ICC, IP & WB.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected phospho Histone H3 (Ser10) in NIH/3T3 cells.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cell lysate.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Physical form
Protein A purified
Purified rabbit conjugate in buffer containing PBS, 15 mg/ml BSA, and 0.1% Sodium Azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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