06-661
Anti-Calreticulin Antibody
Upstate®, from rabbit
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ICC, IHC, WB
Citations:
20
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
pig, human, mouse, Xenopus, bovine, rat
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CALR(811)
General description
60kDa
Immunogen
peptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin
Application
Anti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB.
Research Sub Category
PI3K, Akt, & mTOR Signaling
Enzymes & Biochemistry
PI3K, Akt, & mTOR Signaling
Enzymes & Biochemistry
Biochem/physiol Actions
calreticulin; does not cross react with other endoplasmic reticulum proteins
Physical form
0.1M Tris-glycine, pH 7.0, 0.15M NaCl and 0.05% sodium azide.
Format: Purified
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from murine 3T3/NIH fibroblasts
Other Notes
Replaces: MABT145
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Endoplasmic reticulum form of calreticulin modulates glucocorticoid-sensitive gene expression.
Michalak, M, et al.
The Journal of Biological Chemistry, 271, 29436-29445 (1996)
Calreticulin inhibits glucocorticoid- but not cAMP-sensitive expression of tyrosine aminotransferase gene in cultured McA-RH7777 hepatocytes.
Burns, K, et al.
Molecular and Cellular Biochemistry, 171, 37-43 (1997)
Maria L Valencik et al.
Circulation research, 99(12), 1403-1410 (2006-11-11)
Integrins mechanically link the cytoskeleton to the extracellular matrix in cardiac myocytes and are thereby involved in mechanotransduction. Integrins appear to be necessary for cardiac myocyte hypertrophy. To determine the effect of increased integrin ligation and signaling on adult cardiac