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Merck
CN

06-719

Anti-LexA Antibody, DNA-binding region

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
31

Key Documents

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biological source

rabbit

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

E. coli

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NP_418467.1

UniProt accession no.

P0A7C2

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

Escherichia coli K12 ... Lexa(948544)

General description

24 kDa
The anti-LexA Antibody allows detection of LexA protein and recombinant proteins fused to the LexA protein. This antibody can be used to detect expression of potential bait proteins constructed for use with the yeast two-hybrid system or other interaction trap systems. LexA adds about 22 to 25 kDa to your protein, depending on the number of extra amino acids.

Immunogen

His-tagged DNA binding domain of LexA protein corresponding to amino acid residues 1-202

Application

Detect LexA with Anti-LexA Antibody, DNA-binding region (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.

Biochem/physiol Actions

Recognizes LexA portion of bait fusion proteins used in the yeast two-hybrid system.

Physical form

Format: Purified

Analysis Note

Control
LexA-pRB transfected yeast cell extract
routinely evaluated by immunoblot on LexA-pRB transfected yeast lysate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions.
Hoshino, A; Fujii, H
Journal of Bioscience and Bioengineering null
Direct identification of insulator components by insertional chromatin immunoprecipitation.
Fujita, T; Fujii, H
Testing null
Vanessa C Fernandes et al.
The Journal of biological chemistry, 294(15), 5980-5992 (2019-02-16)
Genetic testing for BRCA1, a DNA repair protein, can identify carriers of pathogenic variants associated with a substantially increased risk for breast and ovarian cancers. However, an association with increased risk is unclear for a large fraction of BRCA1 variants
Getting more from the two-hybrid system: N-terminal fusions to LexA are efficient and sensitive baits for two-hybrid studies.
Beranger, F, et al.
Nucleic Acids Research, 25, 2035-2036 (1997)
Tyrosine residues direct the ubiquitination and degradation of the NY-1 hantavirus G1 cytoplasmic tail.
Erika Geimonen, Imelyn Fernandez, Irina N Gavrilovskaya, Erich R Mackow
Journal of virology null
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