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Merck
CN

06-719

Anti-LexA Antibody, DNA-binding region

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoprecipitation (IP)
western blot
Species reactivity:
E. coli
Citations:
31
Technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
P0A7C2

Key Documents

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Product Name

Anti-LexA Antibody, DNA-binding region, Upstate®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

E. coli

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_418467.1

UniProt accession no.

P0A7C2

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

Escherichia coli K12 ... Lexa(948544)

Analysis Note

Control
LexA-pRB transfected yeast cell extract
routinely evaluated by immunoblot on LexA-pRB transfected yeast lysate

Application

Detect LexA with Anti-LexA Antibody, DNA-binding region (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.

Biochem/physiol Actions

Recognizes LexA portion of bait fusion proteins used in the yeast two-hybrid system.

General description

24 kDa
The anti-LexA Antibody allows detection of LexA protein and recombinant proteins fused to the LexA protein. This antibody can be used to detect expression of potential bait proteins constructed for use with the yeast two-hybrid system or other interaction trap systems. LexA adds about 22 to 25 kDa to your protein, depending on the number of extra amino acids.

Immunogen

His-tagged DNA binding domain of LexA protein corresponding to amino acid residues 1-202

Physical form

Format: Purified

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ngee Lek et al.
EBioMedicine, 36, 401-409 (2018-10-15)
PAIS exhibits a complex spectrum of phenotypes and pubertal outcomes. The paucity of reliable prognostic indicators can confound management decisions including sex-of-rearing. We assessed whether external masculinisation score (EMS) at birth or functional assays correlates with pubertal outcome in PAIS
Genome-wide analysis of the functions of a conserved surface on the corepressor Tup1.
Green, SR; Johnson, AD
Molecular Biology of the Cell null
Yasmine Ligerot et al.
PLoS genetics, 13(12), e1007089-e1007089 (2017-12-09)
Strigolactones (SLs) are well known for their role in repressing shoot branching. In pea, increased transcript levels of SL biosynthesis genes are observed in stems of highly branched SL deficient (ramosus1 (rms1) and rms5) and SL response (rms3 and rms4)
The SOS regulatory system of Escherichia coli.
Little, J W and Mount, D W
Cell, 29, 11-22 (1982)
Tyrosine residues direct the ubiquitination and degradation of the NY-1 hantavirus G1 cytoplasmic tail.
Erika Geimonen, Imelyn Fernandez, Irina N Gavrilovskaya, Erich R Mackow
Journal of virology null
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