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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PPP1R9B(84687)
General description
120 kDa
Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons.
Immunogen
GST fusion protein corresponding to residues 286-390 of rat brain spinophilin/neurabin-II.
Application
Anti-Spinophilin/Neurabin-II Antibody is an antibody against Spinophilin/Neurabin-II for use in IC, IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Spinophilin/Neurabin-II from 500 μg of rat brain microsomal preparation (Catalog # 12-144).
4 μg of a previous lot immunoprecipitated Spinophilin/Neurabin-II from 500 μg of rat brain microsomal preparation (Catalog # 12-144).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Neuronal & Glial Markers
Synapse & Synaptic Biology
Neuronal & Glial Markers
Biochem/physiol Actions
Other species not known.
Specific for spinophilin, Mr 120 kDa. Also recognizes a band which is suspected to be a cleavage product of spinophilin, Mr 95 kDa.
Physical form
Format: Purified
Protein G Purified
Purified rabbit polyclonal IgG in buffer containing 70% storage buffer (0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide) and 30% glycerol. Store at -20°C.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Analysis Note
Control
Brain tissue
Brain tissue
Routinely evaluated by Western Blot on a rat brain microsomal preparation.
Western Blot Analysis:
0.25-1 μg/mL of this lot detected Spinophilin/Neurabin-II in 20 μg of a rat brain microsomal preparation (Catalog # 12-144).
Western Blot Analysis:
0.25-1 μg/mL of this lot detected Spinophilin/Neurabin-II in 20 μg of a rat brain microsomal preparation (Catalog # 12-144).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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