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Sigma-Aldrich

Anti-Casein Kinase 2 Antibody

Upstate®, from rabbit

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Synonym(s):
Anti-CK2A1, Anti-CKII, Anti-Cka1, Anti-Cka2, Anti-OCNDS
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_001895

UniProt accession no.

P68400

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CSN2(1447)

Specificity

Recognizes Casein Kinase 2.

Immunogen

peptide (C-SRARVYTDVNTHRPRE) corresponding to amino acids 7-22 of human Casein Kinase 2

Application

Anti-Casein Kinase 2 Antibody is an antibody against Casein Kinase 2 for use in WB.
Not recommended for IP.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Quality

routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts or A431 cell lysates

Target description

44 kDa

Linkage

Replaces: 04-1129

Physical form

Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

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Hagen Kulbe et al.
Oncotarget, 7(13), 15648-15661 (2016-02-13)
We previously showed how key pathways in cancer-related inflammation and Notch signaling are part of an autocrine malignant cell network in ovarian cancer. This network, which we named the "TNF network", has paracrine actions within the tumor microenvironment, influencing angiogenesis
Protein kinase CK2 ("casein kinase-2") and its implication in cell division and proliferation.
Pinna, L A and Meggio, F
Progress in Cell Cycle Research, 3, 77-97 (1997)
K Willert et al.
The EMBO journal, 16(11), 3089-3096 (1997-06-02)
The dishevelled (dsh) gene of Drosophila melanogaster encodes a phosphoprotein whose phosphorylation state is elevated by Wingless stimulation, suggesting that the phosphorylation of Dsh and the kinase(s) responsible for this phosphorylation are integral parts of the Wg signaling pathway. We
Paula G Ulery et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(19), 5131-5142 (2006-05-12)
The transcription factor DeltaFosB (also referred to as FosB2 or FosB[short form]) is an important mediator of the long-term plasticity induced in brain by chronic exposure to several types of psychoactive stimuli, including drugs of abuse, stress, and electroconvulsive seizures.
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