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Merck
CN

06-913-I

Sigma-Aldrich

Anti-Sin3A Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Paired amphipathic helix protein Sin3a, Histone deacetylase complex subunit Sin3a, Transcriptional corepressor Sin3a, Sin3A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_035508

UniProt accession no.

Q60520

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SAP30(8819)
mouse ... Sin3A(20466)

General description

Paired amphipathic helix protein Sin3a (UniProt Q60520; also known as Histone deacetylase complex subunit Sin3a, mSin3a, Transcriptional co-repressor Sin3a) is encoded by the Sin3A (also known as mSin3A, AW553200, Kiaa4126, mKIAA4126) gene (Gene ID 8819) in murine species. The transcription regulator Sin3a is a scaffold protein that interacts with various proteins to form specialized complexes that mediate different biological processes, including chromatin modification, embryonic development, DNA repair, mitochondrial metabolism, and gene transcription. SIN3a harbors four paired α-helices (PAH), a histone interaction domain, and a highly conserved region. The histone interaction domain mediates HDACs binding, whereas the highly conserved region interacts with proteins necessary for repression, such as the nuclear receptor hormone co-repressor Alien. The four PAHs represent the main interacting domains of SIN3a and mediate the interactions with a wide variety of DNA-binding transcription factors. Members of the Mad family of repressor proteins, for example, specifically interact with the PAH2 through their Sin3 interacting domain (SID). Although widely studied for its involvement in transcriptional repression, Sin3a is shown to be essential for aryl hydrocarbon receptor-mediated induction of gene transcription.
~160 kDa observed

Immunogen

Epitope: N-terminus
KLH-conjugated peptide (KRRLDDQESPVYAAQQRR-C) corresponding to amino acids 2-19 of mouse Sin3A.

Application

Anti-Sin3A Antibody is an antibody against Sin3A Antibody for use in Western Blotting and Immunocytochemistry.
Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected Sin3A in HeLa, A431, HUVEC, and NIH/3T3 cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Sin3A in 10 µg of HeLa nuclear extract.

Other Notes

Concentration: Please refer to lot specific datasheet.
Replaces: 06-913

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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