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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, human, rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HSP90B1(7184)
General description
~94 kDa
Immunogen
Peptide corresponding to an internal sequence from human GRP94.
Application
Anti-Glucose Regulating Protein 94 (GRP94) Antibody detects level of Glucose Regulating Protein 94 (GRP94) & has been published & validated for use in WB & IC.
Immunocytochemistry:
Rabbit anti-GRP94 from a previous lot detected localization of GRP94 (green) in HEK293 cells. Blue staining is DAPI which stains the nucleus of the cell. Previous lots of this antibody showed positive testing for immunocytochemistry on HeLa, COS7 and HEK293 cells.
Optimal working dilutions must be determined by the end user
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Rabbit anti-GRP94 from a previous lot detected localization of GRP94 (green) in HEK293 cells. Blue staining is DAPI which stains the nucleus of the cell. Previous lots of this antibody showed positive testing for immunocytochemistry on HeLa, COS7 and HEK293 cells.
Optimal working dilutions must be determined by the end user
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Biochem/physiol Actions
Glucose regulating protein 94 (GRP94, Endoplasmin).
Other species not tested.
Physical form
Rabbit polyclonal IgG serum in buffer containing 0.02% sodium azide.
Analysis Note
Routinely evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis:
1:500-1:1,500 dilution of this lot detected GRP94 on 10 μg of Jurkat lysates.
Western Blot Analysis:
1:500-1:1,500 dilution of this lot detected GRP94 on 10 μg of Jurkat lysates.
Other Notes
Replaces: AB3545
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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