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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human, bovine, mouse

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002069.5

UniProt accession no.

P63096

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

bovine ... Gnai1(281790)
human ... GNAI1(2770)
mouse ... Gnai1(14677)
rat ... Gnai1(25686)

General description

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).
~40 kDa

Immunogen

Peptide corresponding to amino acids 345-354 of Gi1 and 346-355 of Gi2

Application

Anti-G Protein Giα 1/2 Antibody is an antibody against G Protein Giα 1/2 for use in WB, IH(P).
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of GI ALPHA 1/2 With Citrate pH 6.0 Epitope Retrieval: Rat Cerebellum GI ALPHA 1/2 representative staining pattern/morphology in rat cerebellum. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes G protein subunit αi1.

Physical form

Format: Purified

Preparation Note

Stable at -20°C in undiluted aliquots for up to 12 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G protein Gialpha 1/2 on 10 μg of L6 lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB4067

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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