07-1517
Anti-phospho-IRS-2 (Ser388) Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Insulin receptor substrate 2, IRS-2
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human
Application:
inhibition assay
western blot
western blot
Technique(s):
inhibition assay: suitable (peptide)
western blot: suitable
western blot: suitable
Citations:
5
Uniprot accession no.:
Product Name
Anti-phospho-IRS-2 (Ser388) Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
inhibition assay: suitable (peptide)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer388)
Quality Level
Gene Information
human ... IRS2(8660)
Application
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Anti-phospho-IRS-2 (Ser388) Antibody detects level of phospho-IRS-2 (Ser388) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay.
Peptide Inhibition Assay Analysis: 10 µg from a representative lot blocked IRS-2 on insulin treated HEK293 cells.
Research Category
Signaling
Signaling
Analysis Note
Control
Untreated and insulin treated HEK293 cell lysates
Untreated and insulin treated HEK293 cell lysates
Evaluated by Western Blot in untreated and insulin treated HEK293 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.
Western Blot Analysis: 5 µg/mL of this antibody detected IRS-2 on 10 µg of untreated and insulin treated HEK293 cell lysates.
Biochem/physiol Actions
This antibody recognizes IRS-2 phosphorylated at Ser388.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic beta cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
~160 kDa observed. The calculated molecular weight is 137 kDa, however IRS-2 has been shown as a ~160 kDa band in western blots (Rother, K., et al. (1998). Journal of Biological Chemistry. 273(28):17491–17497.)
Immunogen
Epitope: Phosphorylated Ser388
KLH-conjugated linear peptide corresponding to human IRS-2 phosphorylated Ser388.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
K I Rother et al.
The Journal of biological chemistry, 273(28), 17491-17497 (1998-07-04)
Insulin receptor substrates (IRSs) are tyrosine-phosphorylated following stimulation with insulin, insulin-like growth factors (IGFs), and interleukins. A key question is whether different IRSs play different roles to mediate insulin's metabolic and growth-promoting effects. In a novel system of insulin receptor-deficient
Dahae Lee et al.
Plants (Basel, Switzerland), 11(5) (2022-03-11)
Herein, we determined whether α-Spinasterol, a stigmastane-type phytosterol isolated from Aster pseudoglehnii, potentially impacts glucose uptake and glucose-stimulated insulin secretion in skeletal muscle cells and pancreatic β-cells, respectively. We observed that A. pseudoglehnii and its fractions enhanced glucose uptake, with
Rania Alaaeldin et al.
Molecules (Basel, Switzerland), 26(24) (2021-12-25)
Insulin resistance contributes to several disorders including type 2 diabetes and cardiovascular diseases. Carpachromene is a natural active compound that inhibits α-glucosidase enzyme. The aim of the present study is to investigate the potential activity of carpachromene on glucose consumption
Reduced phosphorylation of brain insulin receptor substrate and Akt proteins in apolipoprotein-E4 targeted replacement mice.
Ong, QR; Chan, ES; Lim, ML; Cole, GM; Wong, BS
Scientific Reports null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service