07-1802
Anti-TAF3 Antibody
serum, from rabbit
Synonym(s):
Transcription initiation factor TFIID subunit 3, 140 kDa TATA box-binding protein-associated factor, TBP-associated factor 3
Transcription initiation factor TFIID 140 kDa subunit, TAF(II)140, TAF140, TAF140, TAFII140
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ChIP, IP
Citations:
2
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse
species reactivity (predicted by homology)
rhesus macaque, guinea pig, human
technique(s)
ChIP: suitable, immunoprecipitation (IP): suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TAF3(83860)
mouse ... Taf3(209361)
General description
TAF3 is a subunit of TFIID that was initially identified through its interaction with a developmentally regulated transcription factor Bric a brac 1 (bab1), which is implicated in pattern formation and tissue morphogenesis in Drosophila. TFIID is a general or basal transcription factor that regulates initiation by allowing RNA polymerase II to recognize a core promotor. TAF3-TBPL2 complexes are found in myoblasts; however, upon differentiation into myotubes, they are replaced by TRF3-TAF3 complexes. Further research is still required to elucidate the roles and the development of the former to the latter complex.
~180 kDa observed.
Immunogen
GST-tagged recombinant protein corresponding to human TAF3.
Application
Chromatin Immunoprecipitation: A representative lot immunoprecipitated TAF3 using chromatin from transfected cells grown under conditions that favor expression from the MyoD:EGFP transgene promoter over expression from the endogenous MyoD promoter.
Detect TAF3 using this Anti-TAF3 Antibody validated for use in Chromatin Immunoprecipitation (ChIP) and Immunoprecipitation.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Physical form
Rabbit polyclonal serum containing with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
C2C12 cell lysate.
C2C12 cell lysate.
Evaluated by Immunoprecipitation in C2C12 cell lysate.
Immunoprecipitation Analysis: A 1:1,000 dilution of this antibody detected TAF3 in 500 µg of C2C12 cell lysate.
Immunoprecipitation Analysis: A 1:1,000 dilution of this antibody detected TAF3 in 500 µg of C2C12 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
New Products: Antibodies, Assays, Small Molecules, Inhibitors, and Proteins
Geyan Wu et al.
EBioMedicine, 71, 103558-103558 (2021-09-15)
Resistance to platinum-based chemotherapy is a major cause of therapeutic failure during the treatment of epithelial ovarian cancer (EOC) patients. Our study aims to elucidate the molecular mechanisms by which ZNF711 down regulation promotes CISPLATIN resistance in EOC. ZNF711 expression
Lixue Cao et al.
Nature communications, 10(1), 3761-3761 (2019-08-23)
The mechanisms underlying how cells subjected to genotoxic stress reestablish reduction-oxidation (redox) homeostasis to scavenge genotoxic stress-induced reactive oxygen species (ROS), which maintains the physiological function of cellular processes and cell survival, remain unclear. Herein, we report that, via a
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 07-1802 | 04053252757990 |