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Merck
CN

07-2101-M

Anti-phospho PIX Antibody (Ser340)

from rabbit, purified by affinity chromatography

Synonym(s):

Rho guanine nucleotide exchange factor (GEF) 7, PAK-interacting exchange factor beta, guanine nucleotide exchange factor 7, rho guanine nucleotide exchange factor 7, SH3 domain-containing proline-rich protein, p85, PAK3, Beta-Pix, P85COOL1, COOL-1, P85,

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About This Item

eCl@ss:
32160702
UNSPSC Code:
12352203
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse
Application:
western blot
Technique(s):
western blot: suitable
Citations:
Uniprot accession no.:
Q9ES28

Key Documents

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Product Name

Anti-phospho PIX Antibody (Ser340), from rabbit, purified by affinity chromatography

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), human (based on 100% sequence homology)

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NP_001106989

UniProt accession no.

Q9ES28

target post-translational modification

phosphorylation (pSer340)

Quality Level

100

Gene Information

human ... PAK3(5063)

Analysis Note

Control
PIX transfected cell lysates
Evaluated by Western Blot in PIX transfected cell lysates.

Western Blot Analysis: A 1:1000 dilution of this antibody detected PIX on 10 µg of Pix transfected cell lysates.

Application

This phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.

Biochem/physiol Actions

This antibody recognizes PIX phosphorylated at Ser340.

General description

p-21-activated kinase (PAK) interacting exchange factor (PIX) is a guanine nucleotide exchange factor. PIX is a necessary component for the recruitment, regulation and activation of Rac1, as it is able to directly bind Rac1 via its SH3 domain. Studies indicate that PIX may also have an active role as a positive regulator of apoptosis, an inducer of membrane ruffling and in cell migration. Along with Rac1, PIX has been shown to regulate and interact with GIT1 and NOD2. Alternative splicing and initiation give rise to 8 known isoforms. Expression of PIX has been localized to the central nervous system. Isoforms B, C and E have highest expression in the testis and brain.
~ 75 kDa

Immunogen

Epitope: Phosphorylated Ser340
KLH-conjugated linear peptide corresponding to mouse PIX phosphorylated at Ser340.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide, 1 mg/mL BSA and 30% glycerol.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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