07-2130
Anti-Ubiquitin Antibody
from rabbit
Synonym(s):
Polyubiquitin-C, Ubiquitin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human, mouse
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... UBC(7316)
General description
Ubiquitin functions as a marker to indicate the fate of other proteins in the cell. It can be attached as a single molecule or as a polyubiquitin chain. Although ubiquitinated proteins are oftentimes tagged for degradation by the 20S proteosome, ubiquitination can also indicate alternative fates depending on the lysine residue that is involved in linking the ubiquitin chain to the target protein.Ubiquitin chains linked by Lys33 to kinase substrates such as AMP-activated protein kinases, (AMPKα1, BRSK1, MARK3, MARK4, and NUAK1), may activate or deactivate these proteins, supporting the role for uniquitin in regulating signaling.
~8 kDa observed. The calculated molecular weight of this protein is 8 kDa, but has been observed at ~24 kDa due to tetramers. (Kvint, K., et al. (2008). Mol Cell.30(4):498-506.)
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Ubiquitin.
Application
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
This Anti-Ubiquitin antibody is validated for use in IP & WB for the detection of the Ubiquitin protein.
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Ubiquitin on 10 µg of HeLa cell lysate.
Biochem/physiol Actions
Broad species reactivity expected based on sequence similarity.
This antibody recognizes Ubiquitin at the N-terminus
Physical form
Affinity purified
Format: Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Serum starved HeLa cell lysate
Serum starved HeLa cell lysate
Evaluated by Immunoprecipitation in serum starved HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg of this antibody detected Ubiquitin on 5 µL of serum starved HeLa cell lysate.
Immunoprecipitation Analysis: 10 µg of this antibody detected Ubiquitin on 5 µL of serum starved HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kristian Kvint et al.
Molecular cell, 30(4), 498-506 (2008-05-24)
The final outcome of protein polyubiquitylation is often proteasome-mediated proteolysis, meaning that "proofreading" of ubiquitylation by ubiquitin proteases (UBPs) is crucial. Transcriptional arrest can trigger ubiquitin-mediated proteolysis of RNA polymerase II (RNAPII) so a UBP reversing RNAPII ubiquitylation might be
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