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Sigma-Aldrich

Anti-Raf-1 Antibody

Upstate®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002880

UniProt accession no.

P04049

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RAF1(5894)

Specificity

Raf-1

Immunogen

peptide corresponding to amino acids 637-648 of human Raf-1.

Application

Anti-Raf-1 Antibody detects level of Raf-1 & has been published & validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
MAP Kinases

Quality

routinely evaluated by immunoblot in RIPA lysates from 3T3/A31 cell lysates

Target description

70kDa

Linkage

Replaces: 04-412

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein A purified

Storage and Stability

2 years at -20degC

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

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Dectin-1 directs T helper cell differentiation by controlling noncanonical NF-kappaB activation through Raf-1 and Syk.
Sonja I Gringhuis,Jeroen den Dunnen,Manja Litjens,Michiel van der Vlist,Brigitte Wevers et al.
Nature Immunology null
Kiril Bidzhekov et al.
Journal of cellular and molecular medicine, 11(6), 1395-1407 (2008-01-22)
Mouse embryonic endothelial progenitor cells (eEPCs) acquire a mature phenotype after treatment with cyclic adenosine monophosphate (cAMP), suggesting an involvement of Raf serine/threonine kinases in the differentiation process. To test this idea, we investigated the role of B-Raf and C-Raf
Design and discovery of small molecules targeting raf-1 kinase.
Lowinger, Timothy B, et al.
Current Pharmaceutical Design, 8, 2269-2278 (2002)
L Lin et al.
Cell death and differentiation, 13(11), 1982-1993 (2006-04-01)
Melanoma differentiation-associated gene-5 (mda-5) was the first molecule identified in nature whose encoded protein embodied the unique structural combination of an N-terminal caspase recruitment domain and a C-terminal DExD/H RNA helicase domain. As suggested by its structure, cumulative evidences documented
Jennifer A Clark et al.
The Journal of biological chemistry, 279(10), 9233-9247 (2003-12-13)
We have reported previously that protein kinase C (PKC) signaling can mediate a program of cell cycle withdrawal in IEC-18 nontransformed intestinal crypt cells, involving rapid disappearance of cyclin D1, increased expression of Cip/Kip cyclin-dependent kinase inhibitors, and activation of
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