07-496-I
Anti-Myeloperoxidase Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Myeloperoxidase, myeloperoxidase heavy chain
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
FACS, WB
Citations:
10
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
flow cytometry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... MPO(4353)
General description
Myeloperoxidase (EC 1.11.2.2; UniProt P05164) is encoded by the MPO gene (Gene ID 4353) in human. Myeloperoxidase/MPO is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a 745 a.a. single chain precursor with a signal peptide sequence (a.a. 1-48), myeloperoxidase is subsequently cleaved into a light chain (a.a.165-278) and a heavy chain (a.a. 279-745). The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. MPO catalyzes hydrogen peroxide-dependent oxidations and the formation of hypochlorous acid that is central to the microbicidal activity of netrophils.
~92/60 kDa (glycosylated pro-form/heavy chain) observed. 83.87/73.85/87.25 kDa (isoform H17/H14/H7 pro-form), 78.94/82.32 kDa (isoform H17/H7 with a.a. 1-48 propeptide removed), 66.45/66.45/69.83 kDa (isoform H17/H14/H7 with a.a. 1-154 removed), 65.48/65.48/68.86 kDa (isoform H17/H7 isoform H17/H14/H7 with a.a. 1-164 removed), 52.51 kDa (heavy chain; a.a. 279-745) calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
KLH-conjugated linear peptide corresponding to a sequence within human myeloperoxidase heavy chain region.
Application
Anti-Myeloperoxidase Antibody, Cat. No. 07-496-I, is a highly specific rabbit polyclonal antibody that targets Myeloperoxidase and has been tested in Flow Cytometry and Western Blotting.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected myeloperoxidase immunoreactivity in 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized HL-60 cells.
Biochem/physiol Actions
The immunogen sequence is present in the pro-fom and the mature forms of myeloperoxidase (without a.a. 1-48, a.a. 1-154, or a.a. 1-164), as well as the cleaved heavy chain (a.a. 279-745), but not light chain (a.a. 165-278).
Analysis Note
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected myeloperoxidase heavy chain in 10 µg of Jurkat cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected myeloperoxidase heavy chain in 10 µg of Jurkat cell lysate.
Other Notes
Concentration: Please refer to lot specific datasheet.
Replaces: 07-496
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Leticija Zlatar et al.
Cell death & disease, 15(7), 548-548 (2024-08-01)
Tuberculosis (TB) remains one of the top 10 causes of death worldwide and still poses a serious challenge to public health. Recent attention to neutrophils has uncovered unexplored areas demanding further investigation. Therefore, the aim of this study was to
Tatiana Reshetnyak et al.
International journal of molecular sciences, 24(11) (2023-06-10)
Neutrophil Extracellular Traps (NETs) have been implicated in systemic lupus erythematosus (SLE) and antiphospholipid syndrome (APS) pathogenesis. The myeloperoxidase-deoxyribonucleic acid (MPO-DNA) complex and nucleosomes are serum markers of NETosis. The aim of this study was to assess these NETosis parameters
Cristina de Diego et al.
Respiratory research, 25(1), 48-48 (2024-01-20)
Neutrophil extracellular traps (NETs) have repeatedly been related to COVID-19 severity and mortality. However, there is no consensus on their quantification, and there are scarce data on their evolution during the disease. We studied circulating NET markers in patients with
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 07-496-I | 04054839090271 |