Anti-p67-phox (not Human) Antibody

Neutrophil cytosol factor 2 (UniProt Q09014; also known as 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, NADPH oxidase subunit 67 kDa, NCF-2, Ncf2, Neutrophil NADPH oxidase factor 2, p67-phox, p47phox) is encoded by the murine Ncf2 (also known as Noxa2, p67phox) gene in murine species (Gene ID 17970). Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors, including p47-phox, p67-phox, p40-phox and Rac. In the resting cell p47phox, p67phox, and p40phox form a large molecular weight cytosolic complex. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane.

~72 kDa observed. Uncharacterized band(s) may appear in some lysates.

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of mouse p67-phox.

Anti-p67-phox (not Human) Antibody is an antibody against p67-phox, tested in mouse and rat, but is not expected to react with human, for use in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p67-phox (rodent) in rat colon and mouse hindbrain tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in C6 and THP-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected p67-phox (rodent) in 10 µg of C6 and THP-1 cell lysate.

Concentration: Please refer to lot specific datasheet.

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