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Sigma-Aldrich

Anti-Estrogen-related Receptor α Antibody

Upstate®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

hamster, mouse, rat, human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

electrophoretic mobility shift assay: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_000125.2

UniProt accession no.

P03372

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... ESR1(2099)

Related Categories

General description

Estrogen-related receptor alpha is a nuclear receptor protein related to the Estrogen receptor. It is reported to interact with estrogen and similar steroids and functions as a transcriptional activator.
This protein is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. In mouse, ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in mouse in brain, spinal cord, pituitary gland and heart.

Specificity

Recognizes Estrogen-related Receptor α.

Immunogen

Ovalbumin-conjugated, synthetic peptide corresponding to amino acids 339-364 of human Estrogen Receptor-Related α

Application

Anti-Estrogen-related Receptor α Antibody is a Rabbit Polyclonal Antibody for detection of Estrogen-related Receptor α also known as ERR alpha & has been tested in EMSA, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Routinely evaluated by immunoblot on RIPA lysates from HeLa nuclear extracts.

Target description

53 kDa

Physical form

Format: Purified
Protein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Regulatory Information

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H Shigeta et al.
Journal of molecular endocrinology, 19(3), 299-309 (1998-02-14)
Estrogen receptor-related orphan receptor alpha 1 is a member of the steroid/thyroid nuclear receptor superfamily. We have previously cloned the human estrogen receptor-related orphan receptor alpha 1 (hERR alpha 1) cDNA and demonstrated that it enhances estrogen responsiveness of the
A novel steroidal inhibitor of estrogen-related receptor alpha (ERRalpha).
Duellman SJ, Calaoagan JM, Sato BG, Fine R, Klebansky B, Chao WR, Hobbs P, Collins N et al.
Biochemical Pharmacology null
Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif.
Tremblay, AM; Wilson, BJ; Yang, XJ; Giguere, V
Molecular Endocrinology null
MiR-137 targets estrogen-related receptor alpha and impairs the proliferative and migratory capacity of breast cancer cells.
Zhao, Y; Li, Y; Lou, G; Zhao, L; Xu, Z; Zhang, Y; He, F
Testing null
S M Kahl et al.
Journal of applied microbiology, 119(6), 1637-1650 (2015-09-19)
To identify the taxonomic differences between phytopathogenic small-spored Alternaria strains isolated from wheat kernels in Germany and Russia by a polyphasic approach. Ninety-five Alternaria (A.) strains were characterized by their colony colour, their three-dimensional sporulation patterns, mycotoxin production and phylogenetic
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