biological source
rabbit
Quality Segment
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer616)
Gene Information
human ... INSR(3643)
General description
165 kDa
Immunogen
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-IRS-1 (Ser616)
Application
Immunoblot Analysis: 0.5 μg/mL dilutions of this antibody detected phospho-IRS1 (Ser616) in RIPA lysates from TPA treated CHO cells transfected with human IRS1.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
anti-phospho-IRS1 (Ser616) Antibody is an antibody against phospho-IRS1 (Ser616) for use in IC & WB.
Biochem/physiol Actions
Human IRS1 (Insulin Receptor Substrate 1) phosphorylated on Serine residue 616. Sequence 100% homologous with mouse, rat, and pig.
Predicted to cross-react with mouse and rat based on 100% sequence homology, but have not been tested.
Physical form
100 μL of affinity purified rabbit polyclonal IgG in PBS (without Mg2+ and Ca2+), pH 7.3, containing 1.0 mg/mL BSA (IgG, protease free), 0.05% sodium azide and 50% glycerol.
Affinity purified
Preparation Note
1 year at -20°C.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 2