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09-454

Sigma-Aldrich

Anti-Dock2 Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
dedicator of cyto-kinesis 2, dedicator of cytokinesis 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, primate, horse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_004946.2

UniProt accession no.

Q8C3J5

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DOCK2(1794)

General description

Dock2 (Dedicator of cytokinesis 2), is a large protein involved in intracellular signaling networks and a member of the DOCK-A subfamily which function as activators of small G proteins. Dock2 specifically activates isoforms of the small G protein Rac and appears to be expressed specifically in leukocytes. These cells express both Rac1 and Rac2 and Dock2 has been shown to bind and promote nucleotide exchange on both of these isoforms. (Nishihara, 1999)

Specificity

09-454 recognizes mouse DOCK2.
Predicted to cross react with human, equine, and primate, based on 93% sequence homology (13/14 aa). The sequence is 85.7% homologous in rat. Reactivity with other species has not been tested.

Immunogen

Epitope: Internal region
KLH-conjugated synthetic peptide near amino acid 1730, within the internal region of mouse DOCK2.

Application

Research Category
Cell Structure
Research Sub Category
Cytoskeleton
Western Blotting; Anti-Dock2 Antibody is an antibody against Dock2 for use in WB
Immunohistchemistry (Frozen sections): A representative lot of this antibody dectected DOCK2 in mouse and human brain frozen tissue sections (Cimino, PJ et al. (2009). Am. J. Pathol. 175(4); 1622-1630)

Quality

Evaluated by Western blot on mouse spleen lysate 0.5 μg/mL of Anti-DOCK2 at 1 mg/mL.

Target description

180 kDa

Physical form

Peptide affinity purified
Purified in PBS containing 0.05% NaN3.

Storage and Stability

Maintain at 2-8°C for up to 1 year from date of receipt.

Analysis Note

Control
Mouse spleen lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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César Nombela-Arrieta et al.
The Journal of experimental medicine, 204(3), 497-510 (2007-02-28)
Recent observations using multiphoton intravital microscopy (MP-IVM) have uncovered an unexpectedly high lymphocyte motility within peripheral lymph nodes (PLNs). Lymphocyte-expressed intracellular signaling molecules governing interstitial movement remain largely unknown. Here, we used MP-IVM of murine PLNs to examine interstitial motility
DOCK2 is a microglial specific regulator of central nervous system innate immunity found in normal and Alzheimer's disease brain.
Cimino, PJ; Sokal, I; Leverenz, J; Fukui, Y; Montine, TJ
The American Journal of Pathology null
Miho Ushijima et al.
Frontiers in immunology, 9, 243-243 (2018-03-06)
A hallmark of humoral immune responses is the production of antibodies. This process involves a complex cascade of molecular and cellular interactions, including recognition of specific antigen by the B cell receptor (BCR), which triggers activation of B cells and
Addendum: defective Dock2 expression in a subset of ASC-deficient mouse lines.
Sirish K Ippagunta,R K Subbarao Malireddi,Patrick J Shaw,Geoffrey A Neale et al.
Nature Immunology null
Kazuhito Gotoh et al.
Blood, 111(6), 2973-2976 (2008-01-17)
The migratory properties of dendritic cells (DCs) are important for their functions. Although several chemokines and their receptors have been implicated in DC migration, the downstream signaling molecules are largely unknown. Here we show that DOCK2, a hematopoietic cell-specific CDM
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