Key Documents

View All Documentation

09-819

Anti-Lin28 Antibody

Name: Anti-Lin28 Antibody
Brand: MM

from rabbit, purified by affinity chromatography

Synonym(s):

Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1

Select a Size

Change View

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

Citations:

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_078950

UniProt accession no.

Q9H9Z2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LIN28A(79727)

Description

General description

Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancer. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

~28 kDa observed

Immunogen

Histidine-tagged recombinant protein corresponding to human Lin28.

Application

Anti-Lin28 Antibody is a Rabbit Polyclonal Antibody for detection of Lin28 also known as Protein lin-28 homolog A & has been validated in WB.

Biochem/physiol Actions

Other homologies: Rat and Mouse 98% and 97% sequence homology

Analysis Note

Control
NT2/D1 cell lyate

Evaluated by Western Blot in NT2/D1 cell lyate.

Western Blot Analysis: 1:5,000 dilution of this antibody detected Lin28 in 10 µg of NT2/D1 cell lysate.

Still not finding the right product?

Explore all of our products under Anti-Lin28 Antibody

— or —

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c