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Merck
CN

09-874

Sigma-Aldrich

Anti-SAP30 (yeast) Antibody

from rabbit

Synonym(s):

Sin3 corepressor complex subunit SAP30, Sin3-associated polypeptide, 30 kDa, Sin3-associated polypeptide, 30kDa, Sin3A-associated protein, 30kDa, histone deacetylase complex subunit SAP30

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

300

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

Saccharomyces cerevisiae

technique(s)

western blot: suitable

NCBI accession no.

NM_003864.3

UniProt accession no.

P38429

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Saccharomyces cerevisiae ... Sap30(855305)

General description

SAP30 (Sin3A-associated protein) is a widely expressed nuclear protein that is functionally conserved between human and yeast. It is a component of the multiprotein histone deacetylase complex containing the transcriptional repressor Sin3, multiple histone deacetylases (HDAC1 and HDAC2), histone binding proteins (RbAp46 and RbAp48) and other polypeptides. SAP30 can recruit the deacetylase complex to promoter DNA in a Sin3 independent manner and is involved in the recruitment of the mSin3 complex to a specific subset of N-CoR corepressor complexes. The latter function suggests a role for SAP30 in N-CoR-mediated repression. Furthermore, SAP30 may be involved in imparting target specificity to the deacetylase complex.
~27 kDa

Immunogen

Epitope: a.a. 141-157
Immunogen was a synthetic peptide corresponding to a.a. 141-157 of yeast SAP30, conjugated to KLH.

Application

Anti-SAP30 (yeast) Antibody is a Rabbit Polyclonal Antibody for detection of SAP30 (yeast) also known as Sin3 corepressor complex subunit SAP30 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology

Biochem/physiol Actions

Not tested in other species.
Recognizes yeast SAP30 at ~27 kDa.

Physical form

Format: Purified
Protein A purified IgG in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% NaN3.

Preparation Note

Stable for 1 year at 2-8°C from date of shipment.

Analysis Note

Control
Yeast extract
Western Blot Analysis:
1 μg/ml of this antibody detected SAP30 in a yeast extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Specific initiation by RNA polymerase I in a whole-cell extract from yeast.
Schultz, M C, et al.
Proceedings of the National Academy of Sciences of the USA, 88, 1004-1008 (1991)
Y Zhang et al.
Molecular cell, 1(7), 1021-1031 (1998-07-04)
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Recently, histone acetylation and deacetylation were found to be catalyzed by structurally distinct, multisubunit complexes that mediate, respectively, activation and repression of transcription. Here, we identify SAP30
C D Laherty et al.
Molecular cell, 2(1), 33-42 (1998-08-14)
The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30

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