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Merck
CN

1.01865

Readybag® Half Fraser Broth with Supplements

acc. ISO 11290, 62 g, irradiated, For the primary selective enrichment of Listeria sp.

Synonym(s):

Demi Fraser broth, Fraser broth primary enrichment, Fraser Broth, Fraser Listeria selective enrichment Broth

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.74

Key Documents

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Agency

APHA
ISO 11133
ISO 11290

Quality Level

200

form

medium granules (dehydrated (DCM))

manufacturer/tradename

ReadyBag®

technique(s)

microbiological culture: suitable

pH

7.0-7.4 (25 °C, 55 g/L in H2O)

solubility

55 g/L

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Listeria spp.

Related Categories

General description

Half FRASER (Demi FRASER) broth has high nutrient content and the large buffer capacity that creates optimum growth conditions for Listeria. Lithium chloride, nalidixic acid, and acriflavine hydrochloride largely inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate enables the detection of the β-D-glucosidase activity by the formation of a black complex of esculetin with iron (III) ions. However, this reaction is not exclusive to Listeria sp. and hence every primary and secondary enrichment in FRASER broth has to be sub-cultured on selective plating media.

Application

Half FRASER (Demi FRASER) broth with supplements is used for the primary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Articles

Types of Media in Microbiology

The different types of culture media, that are used to grow microorganisms in the laboratory for quality control, are classified by several criteria, such as consistency, composition, or selectivity.

Protocols

Listeria: Surviving Bacteria

Listeria is a public health concern, affecting elderly, pregnant, newborns, and immunocompromised individuals.

李斯特菌：不可不防

近年来，李斯特菌病被定为重大的公共健康问题。这种病的主要感染对象是老年人、孕妇、新生儿和免疫功能低下者。

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

Discover the revised EN ISO 11290-1 and EN ISO 11290-2 standards and gain insights into updated detection methods for Listeria monocytogenes and Listeria spp., including enumeration in food, animal feed, and environmental samples. Explore culture media solutions.

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