1.04157
Fluorescein-5-isothiocyanate (FITC)
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About This Item
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
UNSPSC Code:
41116130
NACRES:
NA.21
MDL number:
composition
Dye, ≥95% spectrophotometric assay
Quality Segment
mp
>360 °C
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
SMILES string
S=C=Nc1cc2c(cc1)C3(OC2=O)c4c(cc(cc4)O)Oc5c3ccc(c5)O
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
General description
In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.
Application
for biochemistry and for fluorescence microscopy
Analysis Note
Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
新产品
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