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Phloxin B (C.I. 45410)

Name: Phloxin B (C.I. 45410)
Brand: MM

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₂Br₄Cl₄Na₂O₅

CAS Number:

18472-87-2

Molecular Weight:

829.63

UNSPSC Code:

41116130

NACRES:

NA.21

MDL number:

MFCD00070627

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Properties

form

solid

Quality Level

200

potency

2870 mg/kg LD₅₀, oral (Rat)

composition

Dye, ≥80% gravimetric

pH

9.7 (20 °C, 1 g/L in H₂O)

solubility

100 g/L

bulk density

650 kg/m³

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[Na+].[Na+].Brc1c2c(cc(c1[O-])Br)C4(OC(=O)c5c4c(c(c(c5Cl)Cl)Cl)Cl)c3c(c(c(c(c3)Br)[O-])Br)O2

InChI

1S/C20H4Br4Cl4O5.2Na/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26;;/h1-2,29-30H;;/q;2*+1/p-2

InChI key

OOYIOIOOWUGAHD-UHFFFAOYSA-L

Description

General description

The dry dye Phloxin B (C.I. 45410) for microscopy Certistain^®, is used for the preparation of a staining solution for cytoplasmic staining in histological sections of human origin. It is mainly used in Hemytoxylin and Eosin (H&E) overview staining that is routinely performed in histological laboratories. Eosin and Phloxine bind to the positively charged plasma proteins. The combination of both dyes results in a broader color range in the tissue. As a CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain^®

Analysis Note

Dye content (gravimetrically): ≥ 80 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 546 - 550 nm
Spec. Absorptivity A 1%/1cm (λ_m_a_x; 0.005 g/l; ethanol 50 %): 930 - 1400
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk

WGK 2

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c