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About This Item
Empirical Formula (Hill Notation):
C37H34N2Na2O10S3
CAS Number:
Molecular Weight:
808.85
NACRES:
NA.21
MDL number:
UNSPSC Code:
85151701
form
solid
Quality Segment
potency
>2000 mg/kg LD50, oral (Rat)
composition
Dye, ≥85% spectrophotometric assay
pH
2.7 (20 °C, 10 g/L in H2O)
mp
290 °C (decomposition)
solubility
150 g/L
bulk density
350 kg/m3
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3-,40,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI key
XJBPDZVCYTYRIN-UHFFFAOYSA-L
General description
The dry dye Fast Green FCF (C.I. 42053) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for modified connective tissue staining acc. to van Gieson in histological sections. Further it is applied in histology as counterstaining in e.g. trichrome staining acc. to Masson and additionally for histochemical staining, as well as in Cytology as counterstain instead of light green SF in Papanicolaou′s staining. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.
Application
for microscopy Certistain®
Analysis Note
Dye content (spectrophotometrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 622 - 626 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 1360 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 622 - 626 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 1360 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Hazard Codes
Signal Word
Warning
Precautionary Statements
Hazard Classifications
Muta. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 2
Flash Point (°F)
352.4 °F - closed cup
Flash Point (°C)
178 °C - closed cup
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