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1.07599

Rhodamine B (C.I.45170)

for microscopy

Synonym(s):

Rhodamine B (C.I.45170), Tetraethylrhodamine, Brilliant pink B

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About This Item

Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
81-88-9
Molecular Weight:
479.01
EC Index Number:
201-383-9
UNSPSC Code:
41116130
MDL number:
MFCD00011931
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Quality Level

400

form

solid

potency

>2000 mg/kg LD50, oral (Rat)

composition

Dye, ≥90% spectrophotometric assay

pH

3-4 (20 °C, 10 g/L in H2O)

mp

199-201 °C

solubility

15 g/L

density

1.31 g/cm3 at 20 °C

bulk density

250 kg/m3

εmax

2115-2350 at 550-552 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

General description

Rhodamine B (C.I. 45170) for microscopy, is a solid dye for fluorescence AFB staining of bacteriological smears and histological tissue sections of human origin.The mechanism is a non-specific binding of fluorescence dye on a substrate, similar to the classical Ziehl-Neelsen method, although the heating of the Rhodamine B color solution can be avoided. The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 550 - 552 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2115 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

This item has

Certificates of Analysis (COA)

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