1.08218
Trifluoroacetic acid solution
(25% solution in water) for protein sequenation
Synonym(s):
Trifluoroacetic acid solution, TFA solution, TFA
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About This Item
Linear Formula:
CF3COOH
CAS Number:
Molecular Weight:
114.02
NACRES:
NB.21
UNSPSC Code:
12352106
Beilstein/REAXYS Number:
5446934
MDL number:
Concentration:
24.5-25.5% (trifluoroacetic acid)
Form:
liquid
Quality Level
form
liquid
composition
Water, 74.5-75.5% Karl Fischer
concentration
24.5-25.5% (trifluoroacetic acid)
density
1.12 g/cm3 at 20 °C
storage temp.
15-25°C
SMILES string
OC(=O)C(F)(F)F
InChI
1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
InChI key
DTQVDTLACAAQTR-UHFFFAOYSA-N
Application
Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).
Analysis Note
Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %
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signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1A
Storage Class
8B - Non-combustible, corrosive hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
危险化学品
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Certificates of Analysis (COA)
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Y Chen et al.
Journal of chromatography. A, 1043(1), 9-18 (2004-08-20)
Trifluoroacetic acid (TFA) remains the dominant mobile phase additive for reversed-phase high-performance liquid chromatography (RP-HPLC) of peptides after more than two decades since its introduction to this field. Generally, TFA has been employed in a concentration range of 0.05-0.1% (6.5-13
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1082180050 | 04022536101781 |